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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제6호
- 발행연도
- 2008.1
- 수록면
- 1,040 - 1,043 (4page)
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초록· 키워드
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Klebsiella oxytoca CCUG 15788 is resistant to Ni2+ at a
concentration of 10 mM and grows in an inducible manner
when exposed to lower concentrations of Ni2+. The complete
genomic sequence of a 4.2-kb HindIII-digested fragment
of this strain was determined from genomic DNA. It was
shown to contain four nickel resistance genes (nirA, nirB,
nirC, and nirD) encoding transporter and transmembrane
proteins for nickel resistance. When the plasmid pKOHI4,
encoding nirABCD, was transformed into Escherichia coli
JM109, the cells were able to grow in Tris-buffered mineral
medium containing 3 mM nickel. TnphoA'-1 insertion
mutants in the four nickel genes nirA, nirB, nirC, and nirD
showed nickel sensitivity. The nir genes were heterogeneously
expressed in E. coli, suggesting functional roles of these
genes in nickel resistance.
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참고문헌 (18)
참고문헌 신청
Dong, Q. / 1994 / Czc/Cnr efflux: A threecomponent chemiosmotic antiport pathway with a 12- transmembrane-helix protein. Mol / Microbiol 14 : 185 ~ 187
Grass, G. / 2000 / Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34 / Bacteriol 182 : 1390 ~ 1398
Grass, G. / 2001 / NreB from Achromobacter xylosoxydans 31A is a nickel-induced transporter conferring nickel resistance / Bacteriol 183 : 2803 ~ 2807
Liesegang, H. / 1993 / Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34 / Bacteriol 175 : 767 ~ 778
Park, J. E. / 2003 / Conjugative plasmid mediated inducible nickel resistance in Hafnia alvei 5-5. Int / Microbiol 6 : 57 ~ 64
Grass, G. / 2000 / Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34 / Bacteriol 182 : 1390 ~ 1398
Grass, G. / 2001 / NreB from Achromobacter xylosoxydans 31A is a nickel-induced transporter conferring nickel resistance / Bacteriol 183 : 2803 ~ 2807
Liesegang, H. / 1993 / Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34 / Bacteriol 175 : 767 ~ 778
Park, J. E. / 2003 / Conjugative plasmid mediated inducible nickel resistance in Hafnia alvei 5-5. Int / Microbiol 6 : 57 ~ 64
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