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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제6호
- 발행연도
- 2008.1
- 수록면
- 1,033 - 1,039 (7page)
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A 3-kb HindIII fragment bearing the cry6Aa2 gene and
the adjacent and intergenic regions was cloned from
Bacillus thuringiensis strain YBT-1518. Two open reading
frames (ORFs), namely, orf1 (termed cry6Aa2) and orf2
that were separated by an inverted-repeat sequence were
identified. orf1 encoded a 54-kDa protein that exhibited
high toxicity to the plant-parasitic nematode Meloidogyne
hapla. The orf2 expression product was not detected by
SDS-PAGE, but its mRNA was detected by RT-PCR. The
orf2 coexpressed with orf1 at a high level in the absence of
the inverted-repeat sequence, whereas, the expression
level of orf1 was decreased. When orf2 was mutated, the level
of orf1 expression was enhanced obviously. In conclusion,
the inverted-repeat sequence disturbs orf2 expression, and
the orf2 downregulates orf1 expression. This is an example
of novel negative regulation in B. thuringiensis and a
potential method for enhancing the expression level of cry
genes.
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참고문헌 (23)
참고문헌 신청
Agaisse, H. / 1995 / How does Bacillus thuringiensis produce so much insecticidal crystal protein? / Bacteriol 177 : 6027 ~ 6032
Agaisse, H. / 1996 / STAB-SD: A Shine- Dalgarno sequence in the 5' untranslated region is a determinant of mRNA stability. Mol / Microbiol 20 : 633 ~ 643
Andrup, L. / 1993 / Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation / Bacteriol 175 : 6530 ~ 6536
Arantes, O. / 1991 / Construction of cloning vectors for Bacillus thuringiensis / Gene 108 : 115 ~ 119
Aronson, A. I. / 1971 / Regulation of extracellular protease production in Bacillus cereus T: Characterization of mutants producing altered amounts of protease / Bacteriol 10
Agaisse, H. / 1996 / STAB-SD: A Shine- Dalgarno sequence in the 5' untranslated region is a determinant of mRNA stability. Mol / Microbiol 20 : 633 ~ 643
Andrup, L. / 1993 / Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation / Bacteriol 175 : 6530 ~ 6536
Arantes, O. / 1991 / Construction of cloning vectors for Bacillus thuringiensis / Gene 108 : 115 ~ 119
Aronson, A. I. / 1971 / Regulation of extracellular protease production in Bacillus cereus T: Characterization of mutants producing altered amounts of protease / Bacteriol 10
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