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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제10호
- 발행연도
- 2008.1
- 수록면
- 1,717 - 1,721 (5page)
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초록· 키워드
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Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.
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등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Che, X. Y. / 2005 / Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J. Infect / Dis 191 : 2
Dutta, N. K. / 2008 / Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine / Immunol. Lettl 118 : 65 ~ 71
Huang, L. R. / 2004 / Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA. J. Med / Virol 73 : 338 ~ 346
Marra, M. A. / 2003 / The genome sequence of the SARS-associated coronavirus / Science 300 : 1399 ~ 1404
Reithinger, R. / 2002 / Rapid detection of Leishmania infantum infection in dogs: Comparative study using an immunochromatographic dipstick test, enzyme-linked immunosorbent
Dutta, N. K. / 2008 / Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine / Immunol. Lettl 118 : 65 ~ 71
Huang, L. R. / 2004 / Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA. J. Med / Virol 73 : 338 ~ 346
Marra, M. A. / 2003 / The genome sequence of the SARS-associated coronavirus / Science 300 : 1399 ~ 1404
Reithinger, R. / 2002 / Rapid detection of Leishmania infantum infection in dogs: Comparative study using an immunochromatographic dipstick test, enzyme-linked immunosorbent
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