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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제10호
- 발행연도
- 2008.1
- 수록면
- 1,689 - 1,694 (6page)
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초록· 키워드
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The ability to detect Salmonella spp. is essential in the
prevention of foodborne illness. This study examined a
Salmonella spp. detection method involving the application
of immunomagnetic separation and immunoliposomes
(IMS/IL) encapsulating sulforhodamine B (SRB), a
fluorescent dye. A quantitative assay was conducted by
measuring the fluorescence intensity of SRB that was
produced from an immunomagnetic bead-Salmonella
spp.-immunoliposome complex. The results indicated
detection limits of 2.7×105 and 5.2×103 CFU/ml for Salmonella
enterica subsp. enterica serovar Enteritidis (S. Enteritidis)
and Salmonella enterica subsp. enterica serovar Typhimurium
(S. Typhimurium), respectivley. The signal/noise ratio was
improved by using 4% skim milk as a wash solution
rather than 2% BSA. In addition, higher fluorescence
intensity was obtained by increasing the liposome size.
Compared with the conventional plating method, which
takes 3-4 days for the isolation and identification of
Salmonella spp., the total assay time of 10 h only including
6 h of culture enrichment was necessary for the Salmonella
detection by IMS/IL. These results indicate that the IMS/
IL has great potential as an alternative rapid method for
Salmonella detection.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Bangham, A. D. / 1965 / Diffusion of univalent ions across the lamellae of swollen phospholipids / Mol. Biol 13 : 238 ~ 252
Bautista, D. A. / 2002 / Evaluation of an immunochromatography strip assay for the detection of Salmonella sp. from poultry. J. Vet. Diagn / Invest 14 : 427 ~ 430
Chen, L. / 2007 / Immunomagnetic separation and MS/SPR end-detection combined procedure for rapid detection of Staphylococcus aureus and protein A. Biosens / Bioelectron 22 :
Cudjoe, K. S. / 1991 / Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA. Int. J. Food / Microbio
Davies, C. / 1994 / Technical performance concepts. In D. Wild (ed.), The Immunoassay Handbook / Macmillan Press
Bautista, D. A. / 2002 / Evaluation of an immunochromatography strip assay for the detection of Salmonella sp. from poultry. J. Vet. Diagn / Invest 14 : 427 ~ 430
Chen, L. / 2007 / Immunomagnetic separation and MS/SPR end-detection combined procedure for rapid detection of Staphylococcus aureus and protein A. Biosens / Bioelectron 22 :
Cudjoe, K. S. / 1991 / Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA. Int. J. Food / Microbio
Davies, C. / 1994 / Technical performance concepts. In D. Wild (ed.), The Immunoassay Handbook / Macmillan Press
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