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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제10호
- 발행연도
- 2008.1
- 수록면
- 1,634 - 1,640 (7page)
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This study was focused on the screening of valuable genetic
resources, such as promoters from metagenome, and
describes a promoter trapping system with a bidirectional
probe concept, which can select promoters or operons
from various biological resources including metagenomic
DNA. A pair of reporters, GFP and DsRed, facing the
opposite direction without promoters, is an effective system
that can function regardless of the direction of inserted
promoters. The feasibility of this system was tested for the
isolation of constitutively expressed promoters in E. coli
from a soil metagenome, resulting in a potential pool of
various promoters for practical application. The analyses
of structural organization of the trapped genes demonstrated
that constitutively expressible promoters in E. coli were
broadly distributed within the metagenome, and suggested
that some promoters were useful for the construction of
expression vectors. Based on these observations, three
constitutive promoters were employed in the expression
vector system and their potentials for practical application
were evaluated in terms of expression level, protein solubility,
and effects on host growth.
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등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Amann, R. I. / 1995 / Phylogenetic identification and in-situ detection of individual microbial-cells without cultivation / Microbiol. Rev 59 : 143 ~ 169
Aoki, T. / 2003 / General properties of GFP-display, an electrophoretic analysis for single amino acid changes in target polypeptides / Anal. Biochem 317 : 107 ~ 115
Chen, S. / 2007 / Characterization of strong promoters from an environmental Flavobacterium hibemum strain by using a green fluorescent protein-based reporter system. Appl. E
Crameri, A. / 1996 / Improved green fluorescent protein by molecular evolution using DNA shuffling / Nature Biotechnol 14 : 315 ~ 319
Daniel, R. / 2004 / The soil metagenome-a rich resource for the discovery of novel natural products. Curr. Opin / Biotechnol 15 : 199 ~ 204
Aoki, T. / 2003 / General properties of GFP-display, an electrophoretic analysis for single amino acid changes in target polypeptides / Anal. Biochem 317 : 107 ~ 115
Chen, S. / 2007 / Characterization of strong promoters from an environmental Flavobacterium hibemum strain by using a green fluorescent protein-based reporter system. Appl. E
Crameri, A. / 1996 / Improved green fluorescent protein by molecular evolution using DNA shuffling / Nature Biotechnol 14 : 315 ~ 319
Daniel, R. / 2004 / The soil metagenome-a rich resource for the discovery of novel natural products. Curr. Opin / Biotechnol 15 : 199 ~ 204
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