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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제2호
- 발행연도
- 2009.1
- 수록면
- 167 - 171 (5page)
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초록· 키워드
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The effects of culture conditions on succinic acid production
and its possible scale-up have been studied. Mannheimia
succiniciproducens LPK7, engineered for enhanced production
of succinic acid and reduced by-product secretion, was used
for the experiments. Mannheimia succiniciproducens LPK7
is a knock-out strain of wild type deficient in the ldhA,
pflB, and pta-ackA genes, and is derived from Mannheimia
succiniciproducens MBEL55E. Process optimization of factors
including optimal temperature, pH, carbon source, and
nitrogen source was performed to enhance the production
of succinic acid in flasks. To observe scale-up effects, batch
fermentation was carried out at various working volumes.
At a working volume of 7.0 l, the final succinic acid
concentration and yield were 15.4 g/l and 0.86 g/g. This
result shows similar amount of succinic acid obtained in
lab-scale fermentation, and it is possible to scale up to
larger fermentors without major problems.
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참고문헌 (17)
참고문헌 신청
Agarwal, L. / 2007 / Influence of environmental and nutritional factors on succinic acid production and enzymes of reverse tricarboxylic acid cycle from Enterococcus flavesce
Hong, S. H. / / The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens / Nat. Biotechnol 22 : 1275 ~ 1281
Hong, S. H. / 2002 / Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli / Appl. Microbiol. Biotechnol 58 : 286 ~ 290
Isar, J. / 2006 / Succinic acid production from Bacteroides fragilis: Process optimization and scale up in a bioreactor / Anaerobe 12 : 231 ~ 237
Lee, P. C. / 1999 / Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid production / Process Biochem 35 : 49 ~ 55
Hong, S. H. / / The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens / Nat. Biotechnol 22 : 1275 ~ 1281
Hong, S. H. / 2002 / Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli / Appl. Microbiol. Biotechnol 58 : 286 ~ 290
Isar, J. / 2006 / Succinic acid production from Bacteroides fragilis: Process optimization and scale up in a bioreactor / Anaerobe 12 : 231 ~ 237
Lee, P. C. / 1999 / Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid production / Process Biochem 35 : 49 ~ 55
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