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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제9호
- 발행연도
- 2009.1
- 수록면
- 982 - 986 (5page)
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초록· 키워드
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For the production of ghost bacteria vaccine to prevent
the streptococcal disease in aquaculture fish species, a
double cassettes vector was constructed and cloned in
Escherichia coli DH5α. Ghost bacteria vaccine production
from Escherichia coli DH5α/pHCE-InaN-GAPDH-Ghost
37 SDM (SIG) was maximized at a glucose concentration
of 1 g/l, agitation of 300 rpm, and aeration of 1 vvm. The
maximal efficiency of ghost bacteria formation was
obtained at the mid-exponential phase (OD600=2.0) with
the concentration of 0.77 g/l for SIG. The molecular mass
of GAPDH was detected at 67 kDa with the insoluble
fraction, by SDS-PAGE and Western blot. The protective
efficacy of ghost bacteria vaccine was evaluated by challenge
test using olive flounder. The cumulative mortalities of the
positive control, formalin-killed cell (FKC) vaccine, and
SIG vaccine immunized groups were 91%, 74%, and
57%, respectively. These results suggest that SIG vaccine
showed efficacy as a vaccine and had a higher potential to
induce protective antibodies than did FKC vaccine.
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참고문헌 (22)
참고문헌 신청
Agnew, W. / 2007 / Streptococcus iniae: An aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination / Vet. Microbiol 122 : 1 ~
Bisno, A. L. / 2003 / Autophagy defends cells against invading group A Streptococcus / Lancet Infect. Dis 306 : 1037 ~ 1040
Cummingham, M. / 2000 / Pathogenesis of group A streptococcal infections / Microbiol. Rev 13 : 470 ~ 511
Dubois, M. / 1956 / Colorimetric method for determination of sugars and related substances / Anal. Chem 28 : 350 ~ 356
Eko, F. O. / 1999 / New strategies for combination vaccines based on the extended recombinant bacterial ghost system / Vaccine 17 : 1643 ~ 1649
Bisno, A. L. / 2003 / Autophagy defends cells against invading group A Streptococcus / Lancet Infect. Dis 306 : 1037 ~ 1040
Cummingham, M. / 2000 / Pathogenesis of group A streptococcal infections / Microbiol. Rev 13 : 470 ~ 511
Dubois, M. / 1956 / Colorimetric method for determination of sugars and related substances / Anal. Chem 28 : 350 ~ 356
Eko, F. O. / 1999 / New strategies for combination vaccines based on the extended recombinant bacterial ghost system / Vaccine 17 : 1643 ~ 1649
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