지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제6호
- 발행연도
- 2012.1
- 수록면
- 818 - 825 (8page)
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초록· 키워드
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Keratinases are exciting keratin-degrading enzymes;however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI database was observed. Certain parameters for immobilization of the purified keratinase on chitosan beads were investigated.
The production of the chitosan beads was optimized using factorial design and surface response techniques. The optimum chitosan bead production for protease immobilization was a 20 g/l chitosan solution in acetic acid [1.5% (v/v)],glutaraldehyde ranging from 34 g to 56 g/l, and an activation time between 6 and 10 h. Under these conditions, above 80% of the enzyme was immobilized on the support. The behavior of the keratinase loading on the chitosan beads surface was well described using the Langmuir model.
The maximum capacity of the support (qm) and dissociation constant (Kd) were estimated as 58.8 U/g and 0.245 U/ml,respectively. The thermal stability of the immobilized enzyme was also improved around 2-fold, when compared with that of the free enzyme, after 30 min at 65oC. In addition, the activity of the immobilized enzyme remained at 63.4% after it was reused five times. Thus, the immobilized enzyme exhibited an improved thermal stability and remained active after several uses.
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참고문헌 (24)
참고문헌 신청
Bockle, B. / 1995 / Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530 / Appl. Environ. Microbiol 61 : 3705 ~ 3710
Bolivar, J. M. / 2010 / Heterofunctional supports for one-step purification, immobilization and stabilization of largemultimeric enzymes: Amino-glyoxyl versus amino-epoxy sup
Box, G. E. P. / 1978 / Statistics for Experiments / Wiley
Brandelli, A. / 2005 / Hydrolysis of native proteins by a keratinolytic protease of Chryseobacterium sp / Ann. Microbiol 55 : 47 ~ 50
Brandelli, A. / 2008 / Bacterial keratinases: Useful enzymes for bioprocessing agricultural wastes and beyond / Food Bioprocess Technol 1 : 105 ~ 116
Bolivar, J. M. / 2010 / Heterofunctional supports for one-step purification, immobilization and stabilization of largemultimeric enzymes: Amino-glyoxyl versus amino-epoxy sup
Box, G. E. P. / 1978 / Statistics for Experiments / Wiley
Brandelli, A. / 2005 / Hydrolysis of native proteins by a keratinolytic protease of Chryseobacterium sp / Ann. Microbiol 55 : 47 ~ 50
Brandelli, A. / 2008 / Bacterial keratinases: Useful enzymes for bioprocessing agricultural wastes and beyond / Food Bioprocess Technol 1 : 105 ~ 116
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