지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제5호
- 발행연도
- 2015.1
- 수록면
- 738 - 744 (7page)
이용수
초록· 키워드
오류제보하기
Tuberculosis is one of the most threatening infectious diseases to public health all over the world, for which Mycobacterium tuberculosis (MTB) is the etiological agent of pathogenesis. Ursolic acid (UA) has immunomodulatory function and exhibits antimycobacterial activity. However, the intracellular killing effect of UA has yet to be elucidated. The aim of this study was to evaluate the intracellular killing effect of UA during mycobacterial infection. The intracellular killing activity of UA was evaluated in the macrophage cell line THP-1 by the MGIT 960 system as well as by CFU count. The production of reactive oxygen species (ROS) and the level of nitric oxide (NO) were measured using DCF-DA and Griess reagent, respectively. Phagocytosis was observed by a fluorescence-based staining method, and the colony forming units were enumerated on 7H11 agar medium following infection. In addition, MRP8 mRNA expression was measured by qRT-PCR. UA significantly decreased the number of intracellular Mycobacterium through generation of ROS and NO. In addition, it profoundly activated the phagocytosis process of THP-1 cells during MTB-infection. Furthermore, our data demonstrated that UA activated the phagocytosis process in human monocyte cells through MRP8 induction. These data suggest that UA firmly contributes to the intracellular killing effect of macrophages during mycobacterial infection.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Brinch KS / 2009 / Plectasin shows intracellular activity against Staphylococcus aureus in human THP-1 monocytes and in a mouse peritonitis model / Antimicrob. Agents Chemoth
Checker R / 2012 / Potent anti-inflammatory activity of ursolic acid, a triterpenoid antioxidant, is mediated through suppression of NF-kB, AP-1 and NF-AT / PLoS One 7 : e313
Giacomini E / 2001 / Infection of human macrophages and dendritic cells with mycobacterium tuberculosis induces a differential cytokine gene expression that modulates T cell
Glickman MS / 2001 / Microbial pathogenesis of Mycobacterium tuberculosis: dawn of a discipline / Cell 104 : 477 ~ 485
Gupta D / 2010 / Suppression of TLR2-induced IL-12, reactive oxygen species, and inducible nitric oxide synthase expression by Mycobacterium tuberculosis antigens expressed i
Checker R / 2012 / Potent anti-inflammatory activity of ursolic acid, a triterpenoid antioxidant, is mediated through suppression of NF-kB, AP-1 and NF-AT / PLoS One 7 : e313
Giacomini E / 2001 / Infection of human macrophages and dendritic cells with mycobacterium tuberculosis induces a differential cytokine gene expression that modulates T cell
Glickman MS / 2001 / Microbial pathogenesis of Mycobacterium tuberculosis: dawn of a discipline / Cell 104 : 477 ~ 485
Gupta D / 2010 / Suppression of TLR2-induced IL-12, reactive oxygen species, and inducible nitric oxide synthase expression by Mycobacterium tuberculosis antigens expressed i
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Ursolic Acid Reduces Mycobacterium tuberculosis- Induced Nitric Oxide Release in Human Alveolar A549 cells
Molecules and Cells
2015 .07
-
Anti-Mycobacterial Activity of Tamoxifen Against Drug-Resistant and Intra-Macrophage Mycobacterium tuberculosis
Journal of Microbiology and Biotechnology
2015 .01
-
Apoptotic Effect of Macrophages against Mycobacterium tuberculosis
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .12
-
Characterization of Endoplasmic Reticulum Stress and Apoptosis in Macrophages Infected with Mycobacterium tuberculosis Isolates from Korea Patients
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015 .09
-
Ursolic acid improves the indoxyl sulfate-induced impairment of mitochondrial biogenesis in C2C12 cells
Nutrition Research and Practice
2022 .04
-
Ursolic acid improves the indoxyl sulfate-induced impairment of mitochondrial biogenesis in C2C12 cells
Nutrition Research and Practice
2022 .04
-
Host Immune Pathways to Mycobacterium tuberculosis Infection
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2024 .09
-
Suffocating Mycobacteria: New Approaches for the Treatment of Tuberculosis and Other Mycobacterial Diseases
한국미생물학회 학술대회논문집
2018 .04
-
Neuroprotective effects of urolithin A on H₂O₂-induced oxidative stress-mediated apoptosis in SK-N-MC cells
Nutrition Research and Practice
2020 .02
-
Neuroprotective effects of urolithin A on H₂O₂-induced oxidative stress-mediated apoptosis in SK-N-MC cells
Nutrition Research and Practice
2020 .02
-
A Fractional Extract of Paederia scandens Regulate Intracellular Mycobacterium tuberculosis Growth in Murine Macrophage via Enhanced NF-κB Signal Pathway and Autophagy
한국미생물학회 학술대회논문집
2022 .10
-
Development of Mycobacterium tuberculosis Infection Model Using a Co-culture System with Human Lung Organoids and Macrophages for the Evaluation of Anti-tubercular Drug Efficacy
한국미생물학회 학술대회논문집
2022 .10
-
UAS 기반 대형 폐기물 발생량 측정 가능성 모색
대한원격탐사학회지
2017 .10
-
Mycobacterium tuberculosis Rv21xx Promotes Bacterial Survival within Macrophages
한국미생물학회 학술대회논문집
2017 .04
-
Mycobacterium tuberculosis-infected Macrophage-specific Delivery Peptide Derived by MPT64
한국미생물학회 학술대회논문집
2021 .08
-
Invasion of Mammalian Cells by Rough Variant of Mycobacterium abscessus
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2016 .12
-
Polychlorinated Biphenyl 77, an Endocrine Disruptor, Attenuates Anti-tuberculosis Activity by Elevating Production of the Anti-inflammatory Cytokine IL-10 in Mouse Macrophages during Mycobacterium tuberculosis Infection
한국미생물학회 학술대회논문집
2022 .10
-
Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Je
Journal of Microbiology and Biotechnology
2019 .01
-
CD1b in immature dendritic cells acquires increased phagocytotic function
미생물학회지
2018 .09
-
마이코박테리움을 신속하게 배양할 수 있는 성장 인자에 관한연구
대한임상검사과학회지
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0