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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제12호
- 발행연도
- 2018.1
- 수록면
- 1,982 - 1,991 (10page)
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초록· 키워드
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Ethanol accumulation inhibited the growth of Saccharomyces cerevisiae during wine fermentation. Autophagy and the release of reactive oxygen species (ROS) were also induced under ethanol stress. However, the relation between autophagy and ethanol stress was still unclear. In this study, expression of the autophagy genes ATG1 and ATG8 and the production of ROS under ethanol treatment in yeast were measured. The results showed that ethanol stress very significantly induced expression of the ATG1 and ATG8 genes and the production of hydrogen peroxide (H2O2) and superoxide anion (O2 ·-). Moreover, the atg1 and atg8 mutants aggregated more H2O2 and O2 ·- than the wild-type yeast. In addition, inhibitors of the ROS scavenging enzyme induced expression of the ATG1 and ATG8 genes by increasing the levels of H2O2 and O2 ·-. In contrast, glutathione (GSH) and N-acetylcystine (NAC) decreased ATG1 and ATG8 expression by reducing H2O2 and O2 ·- production. Rapamycin and 3-methyladenine also caused an obvious change in autophagy levels and simultaneously altered the release of H2O2 and O2 ·-. Finally, inhibitors of the mitochondrial electron transport chain (mtETC) increased the production of H2O2 and O2 ·- and also promoted expression levels of the ATG1 and ATG8 genes. In conclusion, ethanol stress induced autophagy which was regulated by H2O2 and O2 ·- derived from mtETC, and in turn, the autophagy contributed to the elimination H2O2 and O2 ·-.
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등록된 정보가 없습니다.
참고문헌 (52)
참고문헌 신청
Kanki T / 2015 / Mitophagy in yeast: molecular mechanisms and physiological role / Biochim. Biophys. Acta 1853:2756~2765
Tsukada M / 1993 / Isolation and characterization of autophagy defective mutants of Saccharomyces cerevisiae / FEBS Lett 333:169~174
Fracchiolla D / 2016 / Mechanism of cargodirected Atg8 conjugation during selective autophagy / Elife 5(pii):e18544
Weiergräber OH / 2017 / Investigating structure and dynamics of Atg8 family proteins / Methods Enzymol 587:115~142
Klionsky DJ / 2008 / Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes / Autophagy 4:151~175
Tsukada M / 1993 / Isolation and characterization of autophagy defective mutants of Saccharomyces cerevisiae / FEBS Lett 333:169~174
Fracchiolla D / 2016 / Mechanism of cargodirected Atg8 conjugation during selective autophagy / Elife 5(pii):e18544
Weiergräber OH / 2017 / Investigating structure and dynamics of Atg8 family proteins / Methods Enzymol 587:115~142
Klionsky DJ / 2008 / Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes / Autophagy 4:151~175
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