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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제3호
- 발행연도
- 2013.1
- 수록면
- 430 - 435 (6page)
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초록· 키워드
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Multidrug resistance, especially multidrug efflux mechanisms that extrude structurally unrelated cytotoxic compounds from the cell by multidrug transporters, is a serious problem and one of the main reasons for the failure of therapeutic treatment of infections by pathogenic microorganisms as well as of cancer cells. Streptococcus mutans is considered one of the primary causative agents of dental caries and periodontal disease, which comprise the most common oral diseases. A fragment of chromosomal DNA from S.
mutans KCTC3065 was cloned using Escherichia coli KAM32 as host cells lacking major multidrug efflux pumps. Although E. coli KAM32 cells were very sensitive to many antimicrobial agents, the transformed cells harboring a recombinant plasmid became resistant to several structurally unrelated antimicrobial agents such as tetracycline, kanamycin, rhodamin 6G, ampicillin,acriflavine, ethidium bromide, and tetraphenylphosphonium chloride. This suggested that the cloned DNA fragment carries a gene encoding a multidrug efflux pump. Among 49 of the multidrug-resistant transformants, we report the functional gene cloning and characterization of the function of one multidrug efflux pump, namely MdeA from S. mutans, which was expressed in E. coli KAM32.
Judging from the structural and biochemical properties,we concluded that MdeA is the first cloned and characterized multidrug efflux pump using the proton motive force as the energy for efflux drugs.
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참고문헌 (21)
참고문헌 신청
Ajdi, D / 2002 / Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen / Proc. Natl. Acad. Sci 99 : 14434 ~ 14439
Berns, K. I / 1965 / Isolation of high molecular weight DNA from Haemophilus influenzae / J. Mol. Biol 11 : 117 ~ 120
Bolhus, H / 1997 / Mechanisms of multidrug transporters / FEMS Microbiol. Rev 21 : 55 ~ 84
Chen, J / 2002 / VmrA, member of a novel class of Na+- coupled multidrug efflux pump from Vibrio parahaemolyticus / J. Bacteriol 184 : 572 ~ 576
Gold, O. G / 1973 / A selective medium for Streptococcus mutans. Arch. Oral Biol. 18 : 357-364 / A selective medium for Streptococcus mutans 18 : 357 ~ 364
Berns, K. I / 1965 / Isolation of high molecular weight DNA from Haemophilus influenzae / J. Mol. Biol 11 : 117 ~ 120
Bolhus, H / 1997 / Mechanisms of multidrug transporters / FEMS Microbiol. Rev 21 : 55 ~ 84
Chen, J / 2002 / VmrA, member of a novel class of Na+- coupled multidrug efflux pump from Vibrio parahaemolyticus / J. Bacteriol 184 : 572 ~ 576
Gold, O. G / 1973 / A selective medium for Streptococcus mutans. Arch. Oral Biol. 18 : 357-364 / A selective medium for Streptococcus mutans 18 : 357 ~ 364
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