지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제3호
- 발행연도
- 2013.1
- 수록면
- 422 - 429 (8page)
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The aim of this study was to investigate the characteristics of canine uropathogenic Escherichia coli (UPEC) and the interaction between canine UPEC and human bladder epithelial cells. Ten E. coli isolates collected from dogs with cystitis were analyzed for antimicrobial resistance patterns, the presence of virulence factors, and biofilm formation. The ability of these isolates to induce cytotoxicity,invade human bladder epithelial cells, and stimulate an immune response was also determined. We observed a high rate of antimicrobial resistance among canine UPEC isolates. All virulence genes tested (including adhesins,iron acquisition, and protectin), except toxin genes, were detected among the canine UPEC isolates. We found that all isolates showed varying degrees of biofilm formation (mean, 0.26; range, 0.07 to 0.82), using a microtiter plate assay to evaluate biofilm formation by the isolates.
Cytotoxicity to human bladder epithelial cells by the canine UPEC isolates increased in a time-dependent manner, with a 56.9% and 36.1% reduction in cell viability compared with the control at 6 and 9 h of incubation, respectively. We found that most canine UPEC isolates were able to invade human bladder epithelial cells. The interaction between these isolates and human bladder epithelial cells strongly induced the production of proinflammatory cytokines such as IL-6and IL-8. We demonstrated that canine UPEC isolates can interact with human bladder epithelial cells, although the detailed mechanisms remain unknown. The results suggest that canine UPEC isolates, rather than dogspecific pathogens, have zoonotic potential.
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참고문헌 (22)
참고문헌 신청
Albiger, B / 2007 / Role of the innate immune system in host defence against bacterial infections: Focus on the Toll-like receptors / J. Intern. Med 261 : 511 ~ 528
Anderson, G. G / 2004 / Intracellular bacterial communities of uropathogenic Escherichia coli in urinary tract pathogenesis / Trends Microbiol 12 : 424 ~ 430
Blango, M. G / 2010 / Persistence of uropathogenic Escherichia coli in the face of multiple antibiotics / Antimicrob. Agents Chemother 54 : 1855 ~ 1863
Clinical and Laboratory Standards Institute / 2004 / Performance standards for antimicrobial susceptibility testing: Fourteenth informational supplement. M100-S14, In National Committee
Farshad, S / 2012 / Microbial susceptibility, virulence factors, and plasmid profiles of uropathogenic Escherichia coli strains isolated from children in Jahrom, Iran / Arch.
Anderson, G. G / 2004 / Intracellular bacterial communities of uropathogenic Escherichia coli in urinary tract pathogenesis / Trends Microbiol 12 : 424 ~ 430
Blango, M. G / 2010 / Persistence of uropathogenic Escherichia coli in the face of multiple antibiotics / Antimicrob. Agents Chemother 54 : 1855 ~ 1863
Clinical and Laboratory Standards Institute / 2004 / Performance standards for antimicrobial susceptibility testing: Fourteenth informational supplement. M100-S14, In National Committee
Farshad, S / 2012 / Microbial susceptibility, virulence factors, and plasmid profiles of uropathogenic Escherichia coli strains isolated from children in Jahrom, Iran / Arch.
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