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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제12호
- 발행연도
- 2015.1
- 수록면
- 1,977 - 1,988 (12page)
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초록· 키워드
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β-1,3-glucanosyltransferases play essential roles in cell wall biosynthesis in yeast. Kluyveromyces lactis has six putative β-1,3-glucanosyltransferase genes. KlGAS1-1 and KlGAS1-2 are homologs of Saccharomyces cerevisiae gene GAS1. RT-qPCR indicated the transcription level of KlGAS1-1 was significantly reduced while heterologous protein (thermostable xylanase B) secretion was enhanced during medium optimization. To evaluate if these two events were related, and to improve xylanase B secretion in K. lactis, we constructed KlGAS1-1 and KlGAS1-2 single deletion strains and double deletion strain, respectively. KlGAS1-1 gene deletion resulted in the highest xylanase B activity among the three mutants. Only the double deletion strain showed morphology similar to that of the GAS1 deletion mutant in S. cerevisiae. The two single deletion strains differed in terms of cell wall thickness and xylanase B secretion. Transcription levels of β-1,3-glucanosyltransferase genes and genes related to protein secretion and transport were assayed. The β-1,3-glucanosyltransferase genes displayed transcription complementation in the cell wall synthesis process. KlGAS1-1 and KlGAS1-2 affected transcription levels of secretion- and transport-related genes. Differences in protein secretion ratio among the three deletion strains were associated with changes of transcription levels of secretion- and transport-related genes. Our findings indicate that KlGAS1-1 deletion is an effective tool for enhancing industrial-scale heterologous protein secretion in K. lactis.
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참고문헌 (24)
참고문헌 신청
Arnold K / 2006 / The SWISSMODEL workspace: a Web-based environment for protein structure homology modelling / Bioinformatics 22 : 195 ~ 201
Backhaus K / 2010 / A systematic study of the cell wall composition of Kluyveromyces lactis / Yeast 27 : 647 ~ 660
Bartkeviciute D / 2004 / Disruption of the MNN10 gene enhances protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae / FEMS Yeast Res 4 : 833 ~ 840
Cabib E / 2013 / How carbohydrates sculpt cells:chemical control of morphogenesis in the yeast cell wall / Nat. Rev. Microbiol 11 : 648 ~ 655
Cline MS / 2007 / Integration of biological networks and gene expression data using Cytoscape / Nat. Protoc 2 : 2366 ~ 2382
Backhaus K / 2010 / A systematic study of the cell wall composition of Kluyveromyces lactis / Yeast 27 : 647 ~ 660
Bartkeviciute D / 2004 / Disruption of the MNN10 gene enhances protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae / FEMS Yeast Res 4 : 833 ~ 840
Cabib E / 2013 / How carbohydrates sculpt cells:chemical control of morphogenesis in the yeast cell wall / Nat. Rev. Microbiol 11 : 648 ~ 655
Cline MS / 2007 / Integration of biological networks and gene expression data using Cytoscape / Nat. Protoc 2 : 2366 ~ 2382
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