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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제6호
- 발행연도
- 2016.1
- 수록면
- 989 - 998 (10page)
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Acidic endo-polygalacturonases are the major part of pectinase preparations and extensively applied in the clarification of fruits juice, vegetables extracts, and wines. However, most of the reported fungal endo-polygalacturonases are active and stable under narrow pH range and low temperatures. In this study, an acidic endo-polygalacturonase (EPG4) was purified and characterized from a mutant strain of Penicillium oxalicum. The N-terminal amino acid sequence of EPG4 (ATTCTFSGSNGAASASKSQT) was different from those of reported endopolygalacturonases. EPG4 displayed optimal pH and temperature at 5.0 and 60-70°C towards polygalacturonic acid (PGA), respectively, and was notably stable at pH 2.2–7.0. When tested against pectins, EPG4 showed enzyme activity over a broad acidic pH range (>15.0% activity at pH 2.2–6.0 towards citrus pectin; and >26.6% activity at pH 2.2–7.0 towards apple pectin). The Km and Vmax values were determined as 1.27 mg/ml and 5,504.6 U/mg, respectively. The enzyme hydrolyzed PGA in endo-manner, releasing oligo-galacturonates from PGA, as determined by TLC. Addition of EPG4 (3.6 U/ml) significantly reduced the viscosity (by 42.4%) and increased the light transmittance (by 29.5%) of the papaya pulp, and increased the recovery (by 24.4%) of the papaya extraction. All of these properties make the enzyme a potential application in the beverage industry.
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참고문헌 (50)
참고문헌 신청
Alkorta I / 1998 / Industrial applications of pectic enzymes: a review / Process Biochem 33:21~28
Bindler F / 1988 / The problem of methanol concentration admissible in distilled fruit spirits / Food Addit. Contam 5:343~351
Celestino KRS / 2006 / Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry / BMC Biochem 7:23
de Maciel MHC / 2014 / Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system / Fluid Phase Equilib 371:125~130
Dinu D / 2007 / Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16 / J. Biotechnol 131:128~137
Bindler F / 1988 / The problem of methanol concentration admissible in distilled fruit spirits / Food Addit. Contam 5:343~351
Celestino KRS / 2006 / Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry / BMC Biochem 7:23
de Maciel MHC / 2014 / Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system / Fluid Phase Equilib 371:125~130
Dinu D / 2007 / Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16 / J. Biotechnol 131:128~137
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