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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제4호
- 발행연도
- 2017.1
- 수록면
- 768 - 774 (7page)
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초록· 키워드
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Horseradish peroxidase (HRP) catalyzes the oxidation of aromatic compounds by hydrogen peroxide via insoluble polymer formation, which can be precipitated from the wastewater. For HRP immobilization, poly(lactic-co-glycolic acid) (PLGA) fine carrier supports were produced by using the Nano Spray Dryer B-90. Immobilized HRP was used to remove the persistent 2,4- dichlorophenol from model wastewater. Both extracted (9-16 U/g) and purified HRP (11- 25 U/g) retained their activity to a high extent after crosslinking to the PLGA particles. The immobilized enzyme activity was substantially higher in both the acidic and the alkaline pH regions compared with the free enzyme. Optimally, 98% of the 2,4-dichlorophenol could be eliminated using immobilized HRP due to catalytic removal and partly to adsorption on the carrier supports. Immobilized enzyme kinetics for 2,4-dichlorophenol elimination was studied for the first time, and it could be concluded that competitive product inhibition took place.
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참고문헌 (22)
참고문헌 신청
Alemzadeh I / 2009 / Phenols removal by immobilized horseradish peroxidase / J. Hazard. Mater 166 : 1082 ~ 1086
Caramori SS / 2004 / Covalent immobilisation of horseradish peroxidase onto poly (ethylene terephthalate)?poly(aniline) composite / Process Biochem 39 : 883 ~ 888
Chan HK / 2011 / Production methods for nanodrug particles using the bottom-up approach / Adv. Drug Deliver. Rev 63 : 406 ~ 416
Chang Q / 2014 / Immobilization of horseradish peroxidase on NH2-modified magnetic Fe3O4/SiO2 particles and its application in removal of 2,4-dichlorophenol / Molecules 19 :
Dahili LA / 2015 / 2,4-Dichlorophenol removal by purified horseradish peroxidase enzyme and crude extract from horsesradish immobilized to nano spray dried ethyl cellulose p
Caramori SS / 2004 / Covalent immobilisation of horseradish peroxidase onto poly (ethylene terephthalate)?poly(aniline) composite / Process Biochem 39 : 883 ~ 888
Chan HK / 2011 / Production methods for nanodrug particles using the bottom-up approach / Adv. Drug Deliver. Rev 63 : 406 ~ 416
Chang Q / 2014 / Immobilization of horseradish peroxidase on NH2-modified magnetic Fe3O4/SiO2 particles and its application in removal of 2,4-dichlorophenol / Molecules 19 :
Dahili LA / 2015 / 2,4-Dichlorophenol removal by purified horseradish peroxidase enzyme and crude extract from horsesradish immobilized to nano spray dried ethyl cellulose p
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