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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제9호
- 발행연도
- 2017.1
- 수록면
- 1,649 - 1,656 (8page)
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In simultaneous saccharification and fermentation (SSF) for production of cellulosic biofuels, engineered Saccharomyces cerevisiae capable of fermenting cellobiose has provided several benefits, such as lower enzyme costs and faster fermentation rate compared with wild-type S. cerevisiae fermenting glucose. In this study, the effects of an alternative intracellular cellobiose utilization pathway—a phosphorolytic pathway based on a mutant cellodextrin transporter (CDT-1 (F213L)) and cellobiose phosphorylase (SdCBP)—was investigated by comparing with a hydrolytic pathway based on the same transporter and an intracellular β-glucosidase (GH1-1) for their SSF performances under various conditions. Whereas the phosphorolytic and hydrolytic cellobiose-fermenting S. cerevisiae strains performed similarly under the anoxic SSF conditions, the hydrolytic S. cerevisiae performed slightly better than the phosphorolytic S. cerevisiae under the microaerobic SSF conditions. Nonetheless, the phosphorolytic S. cerevisiae expressing the mutant CDT-1 showed better ethanol production than the glucose-fermenting S. cerevisiae with an extracellular β-glucosidase, regardless of SSF conditions. These results clearly prove that introduction of the intracellular cellobiose metabolic pathway into yeast can be effective on cellulosic ethanol production in SSF. They also demonstrate that enhancement of cellobiose transport activity in engineered yeast is the most important factor affecting the efficiency of SSF of cellulose.
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참고문헌 (25)
참고문헌 신청
Galazka JM / 2010 / Cellodextrin transport in yeast for improved biofuel production / Science 330:84~86
Ha SJ / 2011 / Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation / Proc. Natl. Acad. Sci. USA 108:504~509
Kim SR / 2012 / Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol / Trends Biotechnol 30:274~282
Jin YS / 2012 / Model-guided strain improvement:Simultaneous hydrolysis and co-fermentation of cellulosic sugars / Biotechnol. J 7:328~329
Kim SR / 2013 / Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism / Biotechnol. Adv 31:851~861
Ha SJ / 2011 / Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation / Proc. Natl. Acad. Sci. USA 108:504~509
Kim SR / 2012 / Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol / Trends Biotechnol 30:274~282
Jin YS / 2012 / Model-guided strain improvement:Simultaneous hydrolysis and co-fermentation of cellulosic sugars / Biotechnol. J 7:328~329
Kim SR / 2013 / Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism / Biotechnol. Adv 31:851~861
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