지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제9호
- 발행연도
- 2011.1
- 수록면
- 885 - 892 (8page)
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초록· 키워드
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The bacterial communities in the intestinal tracts of earthworm were investigated by culture-dependent and -independent approaches. In total, 72 and 55 pure cultures were isolated from the intestinal tracts of earthworms under aerobic and anaerobic conditions, respectively.
Aerobic bacteria were classified as Aeromonas (40%),Bacillus (37%), Photobacterium (10%), Pseudomonas (7%),and Shewanella (6%). Anaerobic bacteria were classified as Aeromonas (52%), Bacillus (27%), Shewanella (12%),Paenibacillus (5%), Clostridium (2%), and Cellulosimicrobium (2%). The dominant microorganisms were Aeromonas and Bacillus species under both aerobic and anaerobic conditions. In all, 39 DNA fragments were identified by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Aeromonas sp. was the dominant microorganism in feeds, intestinal tracts,and casts of earthworms. The DGGE band intensity of Aeromonas from feeds, intestinal tracts, and casts of earthworms was 12.8%, 14.7%, and 15.1%, respectively.
The other strains identified were Bacillus, Clostridium,Enterobacter, Photobacterium, Pseudomonas, Shewanella,Streptomyces, uncultured Chloroflexi bacterium, and uncultured bacterium. These results suggest that PCRDGGE analysis was more efficient than the culturedependent approach for the investigation of bacterial diversity and the identification of unculturable microorganisms.
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참고문헌 (21)
참고문헌 신청
Aira, M / 2005 / Eisenia fetida (Oligochaeta, Lumbricidae) activates fungal growth, triggering cellulose decomposition during vermicomposting / Microb. Ecol 52 : 738 ~ 746
Albano, H / 2008 / Characterization of microbial population of ‘Alheira’ (a traditional Portuguese fermented sausage) by PCR-DGGE and traditional cultural microbiological m
Araya, R / 2003 / Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analys
Clegg, C. D / 2003 / The impact of grassland management regime on the community structure of selected bacterial groups in soils / FEMS Microbiol. Ecol 43 : 263 ~ 270
Cocolin, L. / 2002 / An application of PCR-DGGE analysis to profile the yeast populations in raw milk / Int. Dairy J 12 : 407 ~ 411
Albano, H / 2008 / Characterization of microbial population of ‘Alheira’ (a traditional Portuguese fermented sausage) by PCR-DGGE and traditional cultural microbiological m
Araya, R / 2003 / Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analys
Clegg, C. D / 2003 / The impact of grassland management regime on the community structure of selected bacterial groups in soils / FEMS Microbiol. Ecol 43 : 263 ~ 270
Cocolin, L. / 2002 / An application of PCR-DGGE analysis to profile the yeast populations in raw milk / Int. Dairy J 12 : 407 ~ 411
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