지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제6호
- 발행연도
- 2013.1
- 수록면
- 759 - 765 (7page)
이용수
초록· 키워드
오류제보하기
The gene encoding squalene synthase (SQS) of the lipidproducing heterotrophic microalga Aurantiochytrium sp.
KRS101 was cloned and characterized. The krsSQS gene is 1,551 bp in length and has two exons and one intron.
The open reading frame of the gene is 1,164 bp in length,yielding a polypeptide of 387 predicted amino acid residues with a molecular mass of 42.7 kDa. The deduced krsSQS sequence shares at least four conserved regions known to be required for SQS enzymatic activity in other species.
The protein, tagged with His6, was expressed into soluble form in Escherichia coli. The purified protein catalyzed the conversion of farnesyl diphosphate to squalene in the presence of NADPH and Mg2+. This is the first report on the characterization of an SQS from a Thraustochytrid microalga.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Chang, M. H. / 2008 / The isolation and characterization of Pseudozyma sp. JCC 207, a novel producer of squalene / Appl. Microbiol. Biotechnol 78 : 963 ~ 972
Chen, G. / 2010 / Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp / N. Biotechnol 27 : 382 ~ 389
Gupta, N. / 2012 / Functional characterization and differential expression studies of squalene synthase from Withania somnifera / Mol. Biol. Rep 39 : 8803 ~ 8812
Hong, W. K. / 2011 / Production of lipids containing high levels of docosahexaenoic acid by a newly isolated microalga, Aurantiochytrium sp. KRS101 / Appl. Biochem. Biotechno
Jiang, Y. / 2004 / Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei / J. Agric. Food Chem 52 : 1196 ~ 1200
Chen, G. / 2010 / Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp / N. Biotechnol 27 : 382 ~ 389
Gupta, N. / 2012 / Functional characterization and differential expression studies of squalene synthase from Withania somnifera / Mol. Biol. Rep 39 : 8803 ~ 8812
Hong, W. K. / 2011 / Production of lipids containing high levels of docosahexaenoic acid by a newly isolated microalga, Aurantiochytrium sp. KRS101 / Appl. Biochem. Biotechno
Jiang, Y. / 2004 / Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei / J. Agric. Food Chem 52 : 1196 ~ 1200
이 논문의 저자 정보
최근 본 자료
댓글(0)
0