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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 306 - 311 (6page)
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초록· 키워드
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Despite advances in surgery, radiotherapy, and chemotherapy, the survival of patients with oral squamous cell carcinoma has not significantly
improved over the past several decades. Gene therapy is currently under investigation and shows us new possibility of cancer curing method. This
experiment was undergone to find out the cell growth inhibition effect and evidence of apoptosis by HCCS-1(human cervical cancer suppressor-1),
one of the candidates of tumor suppressor gene, transducted to human oral cancer cell line.
To determine the efficiency of the adenovirus as a gene delivery vector cell line was transducted with LacZ gene and analysed with X-gal staining.
Northern blot was performed to confirm the transfection with HSCC-1 gene and cell viability was assessed by cell cytotoxicity assay using cell count
kit(CCK). To show the evidence of apoptosis, DNA fragmentation assay and flow cytometry(FACS) were performed.
We had successfully construct the recombinant HSCC-1 adenovirus(Ad5CMV-HCCS-1), and importation efficiency was 20% at 2 MOI(multiplicity
of infection), 80% at 20 MOI. Northern blot analysis showed that a single 0.6kb mRNA transcript was expressed in Ad5CMV-HCCS-1 transducted
cell lines. As a result of CCK, when comparing to control subjects, transducted group showed 50% growth inhibition. In DNA fragmentation assay,
according to increasing of MOI, DNA volume was diminished. In FACS analysis, DNA distribution showed fragmentation.
This results imply that HCCS-1gene has growth inhibition effect in human oral cancer cell lines through apoptosis induction.
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등록된 정보가 없습니다.
참고문헌 (10)
참고문헌 신청
/ 1991 / Mechanism and function of cell death 7 : 663 ~ 698
/ 1993 / Stable expression of thewild-type p53 gene in human lung cancer cells after retrovirus-medi-ated gene transfer 4 : 617 ~ 624
/ 1994 / Regulation of apoptosis inthe immune system 6 : 279 ~ 289
/ 1995 / Apoptosis induced in humanosteosarcoma cells is one of the mechanisms for the cytocidal effectof Ad5CMV-p53 2 : 9 ~ 17
/ 1998 / Cancer gene therapy 7 : 421 ~
/ 1993 / Stable expression of thewild-type p53 gene in human lung cancer cells after retrovirus-medi-ated gene transfer 4 : 617 ~ 624
/ 1994 / Regulation of apoptosis inthe immune system 6 : 279 ~ 289
/ 1995 / Apoptosis induced in humanosteosarcoma cells is one of the mechanisms for the cytocidal effectof Ad5CMV-p53 2 : 9 ~ 17
/ 1998 / Cancer gene therapy 7 : 421 ~
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