지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 63 - 70 (8page)
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초록· 키워드
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Objectives: The purpose of this study was to investigate the wound healing effect of primary cultured oral mucosal keratinocytes (OMKs) and to assess their roles in skin wounds.
Materials and Methods: OMK labeled with BromodeoxyUridine were scattered onto 1.5×1.5 cm skin defects of adult female nude mice (OMK group, n=15). For the control, culture media were placed on the wound (control group, n=15). Mice in both groups were sacrificed at three days (n=5), one week (n=5), and two weeks (n=5), and histomorphometric and immunoblot analyses with keratinocyte growth factor (KGF), interleukin (IL)-6, and IL-1α antibody were performed for the biopsied wound specimen. To verify the effect of the cytokine, rhIL-1α was applied instead of OMK transplantation, and the OMK and control groups were compared with regard to re-epithelialization.
Results: Histomorphometric analyses demonstrated faster re-epithelialization in the graft group than in the control group at the third day, first week, and second week. Newly forming epithelium showed maintenance of the histological character of the skin epithelium. The graft group showed superior expression of KGF, IL-6, and IL-1α protein, compared with the control group. Similar faster re-epithelialization was observed after treatment with rhIL-1α instead of OMK transplantation.
Conclusion: We successfully confirmed that the graft of primary cultured OMKs promoted regeneration of skin defects. The mechanism of accelerated wound healing by primary cultured OMKs was attributed to inducement of cytokine expression as required for re-epithelialization.
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참고문헌 (24)
참고문헌 신청
Andreadis ST / 2001 / Keratinocyte growth factor induces hyperproliferation and delays differentiation in a skin equivalent model system / Faseb J 15 : 898 ~ 906
Cha IH / 2000 / Three dimensional reconstitution of oral mucosal keratinocytes and its biologic characteristics / Korean J Pathol 34 : 181 ~ 189
Cooper ML / 1993 / Direct comparison of a cultured composite skin substitute containing human keratinocytes and fibroblasts to an epidermal sheet graft containing human kera
Eaglstein WH / 1998 / Tissue engineering for skin: an update / J Am Acad Dermatol 39 : 1007 ~ 1010
Gottlieb AB / 1988 / Detection of transforming growth factor alpha in normal, malignant, and hyperproliferative human keratinocytes / J Exp Med 167 : 670 ~ 675
Cha IH / 2000 / Three dimensional reconstitution of oral mucosal keratinocytes and its biologic characteristics / Korean J Pathol 34 : 181 ~ 189
Cooper ML / 1993 / Direct comparison of a cultured composite skin substitute containing human keratinocytes and fibroblasts to an epidermal sheet graft containing human kera
Eaglstein WH / 1998 / Tissue engineering for skin: an update / J Am Acad Dermatol 39 : 1007 ~ 1010
Gottlieb AB / 1988 / Detection of transforming growth factor alpha in normal, malignant, and hyperproliferative human keratinocytes / J Exp Med 167 : 670 ~ 675
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