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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 352 - 357 (6page)
이용수
초록· 키워드
오류제보하기
Background: Malassezia yeasts are normal flora of the skin
found in 75∼98% of healthy subjects. The accurate identification
of the Malassezia species is important for determining
the pathogenesis of the Malassezia yeasts with regard
to various skin diseases such as Malassezia folliculitis,
seborrheic dermatitis, and atopic dermatitis. Objective: This
research was conducted to determine a more accurate and
rapid molecular test for the identification and classification of
Malassezia yeasts. Methods: We compared the accuracy and
efficacy of restriction fragment length polymorphism (RFLP)
and the nested polymerase chain reaction (PCR) for the
identification of Malassezia yeasts. Results: Although both
methods demonstrated rapid and reliable results with regard
to identification, the nested PCR method was faster. However,
7 different Malassezia species (1.2%) were identified
by the nested PCR compared to the RFLP method.
Conclusion: Our results show that RFLP method was relatively
more accurate and reliable for the detection of various
Malassezia species compared to the nested PCR. But, in the
aspect of simplicity and time saving, the latter method has its
own advantages. In addition, the 26S rDNA, which was
targeted in this study, contains highly conserved base
sequences and enough sequence variation for inter-species
identification of Malassezia yeasts.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Bernier V / 2002 / Skin colonization by Malassezia species in neonates: a prospective study and relationship with neonatal cephalic pustulosis / Arch Dermatol 138 : 215 ~ 21
Gaitanis G / 2006 / Distribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. gl
Gandra RF / 2006 / Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients / Mycopathologia 162 : 273 ~ 2
Gemmer CM / 2002 / Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff m
Gueho E / 1996 / The genus Malassezia with description of four new species / Antonie Van Leeuwenhoek 69 : 337 ~ 355
Gaitanis G / 2006 / Distribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. gl
Gandra RF / 2006 / Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients / Mycopathologia 162 : 273 ~ 2
Gemmer CM / 2002 / Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff m
Gueho E / 1996 / The genus Malassezia with description of four new species / Antonie Van Leeuwenhoek 69 : 337 ~ 355
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