지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
A disposable electrochemical immunosensor system has been developed for the detection of herbicide in aqueous samples. Disposable screen printed carbon electrodes(SPCE) were used as basic electrodes and an enzyme, horseradish peroxidase (HRP), and anti-herbicide antibodies was immobilised on to the working electrode of SPCE by using avidin-biotin coupling reactions. An herbicide-glucose oxidase conjugates have been used for the competitive immunoreaction with sample herbicides. The enzymatic reaction between the conjugated glucose oxidase and glucose added generates hydrogen peroxide, which was reduced by the peroxidase immobilised. The latter process caused an electrical current change, due to direct re-reduction of peroxidase by a direct electron transfer mechanism, which was measured to determine the herbicides in the sample. The optimal operational condition was found to be: 20 μgl-1 deglycosylated avidin loading to the working electrode and working potential +50 mV vs. Ag/AgCl. The total assay time was 15 min after sample addition. The detection limits for herbicides, atrazine and simazine, were found to be 3 ppb and 10 ppb, respectively.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
80/778/EEC / 1980 / Council directive relating to the quality of water intended for human consumption
A. Warsinke / 2000 / Electrochemical immunoassays / Fresenius J. Anal.Chem 366 : 622 ~ 634
AOAC / 2010 / Association of Agricultural Chemists official method 974.27, Official methods of analysis of AOAC international / AOAC
B. Dzantiev / 2004 / Determination of the herbicide chlorsulfuron by amperometric sensor based on separation-free bienzyme immunoassay / Sens. Actuat. B 98 : 254 ~ 261
B.Johnson / 2000 / A review of health-based comparative risk assessments in the United States / Rev. Environ. Health 15 : 273 ~ 287
A. Warsinke / 2000 / Electrochemical immunoassays / Fresenius J. Anal.Chem 366 : 622 ~ 634
AOAC / 2010 / Association of Agricultural Chemists official method 974.27, Official methods of analysis of AOAC international / AOAC
B. Dzantiev / 2004 / Determination of the herbicide chlorsulfuron by amperometric sensor based on separation-free bienzyme immunoassay / Sens. Actuat. B 98 : 254 ~ 261
B.Johnson / 2000 / A review of health-based comparative risk assessments in the United States / Rev. Environ. Health 15 : 273 ~ 287
이 논문의 저자 정보
최근 본 자료
댓글(0)
0