지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Plant regeneration protocols via callus induction from seeds of Miscanthus sinensis, a promising bioenergy crop, were developed. Basal media and 2,4-D concentrations, proline concentrations, and kinds and concentrations of gelling agent were tested to find out the optimal conditions for callus induction from mature seeds. On the combination treatment of basal media,MS medium and N6 medium, and 2,4-D concentrations, callus induction rate was the highest (42.2%) in MS medium with 4.0mg/L 2,4-D. Proline improved callus formation from seeds, and high concentration (12.0 g/L) of proline was good for callus induction. In gelling agent treatment, Phytagel was better for callus formation than agar and 2.25 g/L Phytagel produced the highest (86.1%) callus induction. Calli obtained from seeds were subcultured every 4 weeks. After 3 months of callus induction,calli with dense yellow color and dome shape were selected and used for plant regeneration. The callus, cultured on hormone-free MS medium or MS medium with 1.0 mg/L BA, produced adventitious shoot. The regenerated shoots were elongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants showed normal phenotypes.
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참고문헌 (25)
참고문헌 신청
Armstrong CL / 1985 / Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline / Planta 164 : 207 ~ 214
Armstrong CL / 1994 / Regeneration of plants from somatic cell cultures: applications for in vitro genetic manipulation, In The Maize Handbook / Springer Verlag : 663 ~ 671
Beale CV / 1996 / Leaf photosynthesis in the C4-grass Miscanthus giganteus, growing in the cool temperate climate of southern England / J. Exp. Bot 47 : 267 ~ 273
Chris S / 2010 / Feedstocks for lignocellulosic biofuels / Science 329 : 790 ~ 792
Chu CC / 1975 / Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources / Sci. Sin 18 : 659 ~ 668
Armstrong CL / 1994 / Regeneration of plants from somatic cell cultures: applications for in vitro genetic manipulation, In The Maize Handbook / Springer Verlag : 663 ~ 671
Beale CV / 1996 / Leaf photosynthesis in the C4-grass Miscanthus giganteus, growing in the cool temperate climate of southern England / J. Exp. Bot 47 : 267 ~ 273
Chris S / 2010 / Feedstocks for lignocellulosic biofuels / Science 329 : 790 ~ 792
Chu CC / 1975 / Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources / Sci. Sin 18 : 659 ~ 668
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