지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.
목차
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참고문헌 (17)
참고문헌 신청
Baker WJ / 2000 / Molecular Phylogenetics of Calamus (Palmae) and Related Rattan Genera Based on 5S nrDNA Spacer Sequence Data / Mol. Phylogenet Evol 14 (2) : 218 ~ 231
Baum BR / 1994 / The molecular diversity of the 5S rRNA gene in barley (Hordeum vulgare) / Genome 37 : 992 ~ 998
Baum BR / 2004 / The utility of the nontranscribed spacer of 5S rDNA grouped into unit classes assigned to haplomes - a test on cultivated wheat and wheat progenitors / Genom
Baum BR / 2007 / The 5S DNA sequences in Hordeum bogdanii and in the H. brevisubulatum complex, and the evolution and the geographic dispersal of the diploid Hordeum species
Fulnecek J / 2006 / Plant 5S rDNA has multiple alternative nucleosome positions / Genome 49 (7) : 840 ~ 850
Baum BR / 1994 / The molecular diversity of the 5S rRNA gene in barley (Hordeum vulgare) / Genome 37 : 992 ~ 998
Baum BR / 2004 / The utility of the nontranscribed spacer of 5S rDNA grouped into unit classes assigned to haplomes - a test on cultivated wheat and wheat progenitors / Genom
Baum BR / 2007 / The 5S DNA sequences in Hordeum bogdanii and in the H. brevisubulatum complex, and the evolution and the geographic dispersal of the diploid Hordeum species
Fulnecek J / 2006 / Plant 5S rDNA has multiple alternative nucleosome positions / Genome 49 (7) : 840 ~ 850
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