지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Molecular genetic markers have been widely used as powerful tools for analyzing the genome. In particular, SSR markers have practical applications in breeding systems because they can be used in high-throughput analyses for genetic mapping, heritable diversity testing, purity analysis, and marker-assisted selection. Currently, due to technical advances in DNA sequencing, large sequence databases are available for large-scale SSR mining and marker development. Here, we describe an automated method, the SSR Finder program, for SSR discovery in the onion sequence database, and primer design for amplifying the detected SSRs. A total of 1,049 SSR primers were obtained for genetic purity testing, and 100 SSR sets were analyzed in 14 bulb onion breeding lines using clustering analysis. A total of 20 selected markers from screening of all 1,049 SSR primers,were finally applied for genetic purity testing in three breeding lines, NW1, NW9, and NW10. The initial tests showed that 15%,71%, and 97% of individuals within NW1, NW9, and NW10, respectively, were genetically homogeneous. These markers produced using the SSR Finder will be useful for investigating the genetic purity of onion breeding lines.
목차
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참고문헌 (25)
참고문헌 신청
Anderson JA / 1993 / Optimizing parental selection for genetic linkage maps / Genome 36 : 181 ~ 186
Arumuganathan K / 1991 / Nuclear DNA content of some important plant species / Plant Mol. Biol. Rep 9 : 208 ~ 218
Bradeen JM / 1995 / Randomly amplified polymorphic DNA in bulb onion and its use to assess inbred integrity / J. Am. Soc. Hort. Sci 120 : 752 ~ 758
Bredemeijer GMM / 1998 / The use of semi-automated fluorescent microsatellite analysis for tomato cultivar identification / Theor. Appl. Genet 97 : 584 ~ 590
Fischer D / 2000 / Onion microsatellite for germplasm analysis and their use in assessing intra- and inter-specific relatedness within the subgenus Rhizirideum / Theor. Appl.
Arumuganathan K / 1991 / Nuclear DNA content of some important plant species / Plant Mol. Biol. Rep 9 : 208 ~ 218
Bradeen JM / 1995 / Randomly amplified polymorphic DNA in bulb onion and its use to assess inbred integrity / J. Am. Soc. Hort. Sci 120 : 752 ~ 758
Bredemeijer GMM / 1998 / The use of semi-automated fluorescent microsatellite analysis for tomato cultivar identification / Theor. Appl. Genet 97 : 584 ~ 590
Fischer D / 2000 / Onion microsatellite for germplasm analysis and their use in assessing intra- and inter-specific relatedness within the subgenus Rhizirideum / Theor. Appl.
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