지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Blueberry (Vaccinium spp.) is a member of Ericaceae and the recent important small fruit crop. This study was toconstruct a DNA marker database for blueberry varieties in Korea using simple sequence repeat (SSR) markers. A set of 49 SSRprimer pairs was tested to select polymorphic SSR marker in 6 varieties. With 49 primer pairs, seventeen primer pairs showedpolymorphism, reproducibility and band clearance. The genetic relationship among 34 varieties by using 17 SSR markers wasanalyzed. A total of 115 polymorphic amplified fragments were obtained by 17 SSR markers. Two to fifteen SSR alleles weredetected for each locus with an average of 6.8 alleles per locus. Average polymorphism information content (PIC) was 0.671,ranging from 0.248 to 0.888. A total of 115 SSR marker loci were used to calculate Jaccard’s distance coefficients for clusteranalysis by unweighted pair-group method with arithmetical average (UPGMA). Genetic distance ranged from 0.31 to 0.81 in34 varieties and the dendrogram at a similarity 0.40 gave 3 main clusters according to blueberry species. Totally 34 varietieswere identified by 17 SSR markers. Out of 17 SSR markers, a set of 3 minimum SSR markers was also enabled the identificationof 34 varieties. Hence, we concluded that these SSR markers will be available for identifying blueberry varieties and alternativechoice to distinctness, uniformity, stability (DUS) examination in blueberry along with valid phenotypic data.
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참고문헌 (24)
참고문헌 신청
Agawal M / 2008 / Advances in molecular marker techniques and their applications in plant sciences / Plant Cell Reports 27 : 617 ~ 631
Anderson JA / 1993 / Optimizing parental selection for genetic linkage maps / Genome 36 : 181 ~ 186
Aruna M / 1995 / Randomly amplified polymorphic DNA fingerprinting for identifying rabbiteye blueberry (Vaccinium ashei Reade) cultivars / J. Am. Soc. Hortic. Sci 120 : 710 ~
Bell DJ / 2008 / Suitability of EST-PCR markers developed in highbush blueberry for genetic fingerprinting and relationship studies in lowbush blueberry and related species /
Bidani A / 2013 / An economical molecular tool for genetic identity confirmation in blueberry / American Society Horticultural Science Annual Conference
Anderson JA / 1993 / Optimizing parental selection for genetic linkage maps / Genome 36 : 181 ~ 186
Aruna M / 1995 / Randomly amplified polymorphic DNA fingerprinting for identifying rabbiteye blueberry (Vaccinium ashei Reade) cultivars / J. Am. Soc. Hortic. Sci 120 : 710 ~
Bell DJ / 2008 / Suitability of EST-PCR markers developed in highbush blueberry for genetic fingerprinting and relationship studies in lowbush blueberry and related species /
Bidani A / 2013 / An economical molecular tool for genetic identity confirmation in blueberry / American Society Horticultural Science Annual Conference
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