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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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To identify and apply tissue-specific promoters is one of the major challenges in plants genetic engineering for optimizingefficient expression of interest genes in appropriate tissues. In this research, open-source database information of Arabidopsisthaliana was adapted to determine root-specific expressed promoter region. A total seven sequences that might function as aroot-specific promoter element were initially isolated from Arabidopsis genomic DNA. Then seven promoters were cloned intopBGWFS7 in which β-glucuronidase (gus) and green fluorescent protein (gfp) genes were linked. The GUS activities weremeasured in different tissues of transgenic Arabidopsis by both histochemical GUS staining and fluorescent 4 methylumbelliferylβ-D-glucuronide (MUG) assay. To confirm root-specific expression, GFP-confocal microscope analysis was conducted in Arabidopsistransgenic plant. As a result, the five promoters showed strong GUS activity in the root tissue as compared with the CaMV35Spromoter. To test crop application availability as a root-specific promoter, seven promoters were introduced into tomato plantsand confirmed transient expression using Agrobacterium rhizogenesis ARqua1 root-nodule inducible strain. Two promoters showedthat gus genes were specifically expressed in roots of transgenic tomato plant. Taken together, the novel seven promoters showedspecific activity in root suggesting that it is applicable in crop improvement.
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참고문헌 (39)
참고문헌 신청
Ali MA / 2015 / Plant nematode interactions: from genomics to metabolomics / Int J Agric Biol 17:1071~1082
Brisson N / 1984 / Expression of a bacterial gene in plants by using a viral vector / Nature 310:511~514
Bucher M / 2002 / The expression of an extensin-like protein correlates with cellular tip growth in tomato / Plant Physiol 128:911~923
Cheon BY / 2004 / Overexpression of human erythropoietin (EPO) affects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis / Transgenic Res 13:541~549
Cho HT / 2002 / Regulation of root hair initiation and expansin gene expression in Arabidopsis / Plant Cell 14:3237~3253
Brisson N / 1984 / Expression of a bacterial gene in plants by using a viral vector / Nature 310:511~514
Bucher M / 2002 / The expression of an extensin-like protein correlates with cellular tip growth in tomato / Plant Physiol 128:911~923
Cheon BY / 2004 / Overexpression of human erythropoietin (EPO) affects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis / Transgenic Res 13:541~549
Cho HT / 2002 / Regulation of root hair initiation and expansin gene expression in Arabidopsis / Plant Cell 14:3237~3253
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