개인구독
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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The color of wheat flour and dough affect significantly on the quality of wheat. The major factor causing darkening of dough color is activity of polyphenol oxidase (PPO). Therefore, we examined the relationship between the PPO activities and the genotypes of the PPO-specific molecular markers in Korean wheat cultivars, and tested the usefulness of the selected markers for the selection of Korean wheat germplasm lines low in PPO activity. Five sequence-tagged site (STS) markers specific to wheat PPO genes, PPO-05, PPO-16, PPO-18, PPO-30 and PPO-43, were examined for their levels of polymorphism in Korean wheat cultivars. The genotypes of the marker PPO-18, which is linked to Ppo-A1 locus, were correlated significantly highly with the levels of grain PPO activity. Furthermore, Korean wheat cultivars could be classified into the high or low PPO activity group by the allele type of Ppo-A1 which was differentiated by the PPO-18. Grain PPO activity was lower about twice in the cultivars with Ppo-A1b allele than in those with Ppo-A1a allele. The cultivars with Ppo-A1b allele had lower grain weight and wide grain width, also they had less ash, protein, Fe and total polyphenol than those with Ppo-A1a allele. Thus, the lightness of flour and dough sheet was brighter in the cultivars with Ppo-A1b allele. The four wheat cultivars including Suwon252, which had lowest PPO activity and Ppo-A1b allele, were selected from 200 genetic lines as valuable genetic resources for the breeding of wheat variety with bright white-colored flour.
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참고문헌 (21)
참고문헌 신청
AACC / 2000 / Approved methods of the American Association of Cereal Chemists, 9th edn / AACC
Anderson JV / 2001 / An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity / Crop Sci 41 : 1697 ~ 1705
Baik BK / 1994 / Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles / Cereal Chem 71 : 315 ~ 320
Baik BK / 1995 / Discoloration of dough for oriental noodles / Cereal Chem 72 : 198 ~ 205
Cary JW / 1992 / Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase / Plant Mol. Biol 20 : 245 ~ 253
Anderson JV / 2001 / An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity / Crop Sci 41 : 1697 ~ 1705
Baik BK / 1994 / Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles / Cereal Chem 71 : 315 ~ 320
Baik BK / 1995 / Discoloration of dough for oriental noodles / Cereal Chem 72 : 198 ~ 205
Cary JW / 1992 / Cloning and characterization of cDNAs coding for Vicia faba polyphenol oxidase / Plant Mol. Biol 20 : 245 ~ 253
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