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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 269 - 274 (6page)
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DNA methyltransferases(DNMTs) are closely associated with the epigenetic change and the gene silencing through the regulation of methylation status in animal genome. But, the role of DNMTs in transgene silencing has remained unclear. So, we examined whether the knockdown of DNMT influences the reactivation of transgene expression in the transgenic quails. In this study, we investigated the expression of DNMT3a, and DNMT3b in blastoderm, quail embryonic fibroblasts(QEFs) and limited embryonic tissues such as gonad, kidney, heart and liver of E6 transgenic quails(TQ2) by RT-PCR. We further analyzed the expression of DNMT3a at different stages of whole embryos during early embryonic development by qRT-PCR. DNMT3a expression was detected in all test samples; however, it showed the highest expression in E6 whole embryo. Embryonic fibroblasts collected from TQ2 quails were treated with two DNMT3a-targeted siRNAs(siDNMT3a-51 and siDNMT3a-88) for RNA interference assay, and changes in expression were then analyzed by qRT-PCR. The siDNMT3a-51 and siDNMT3a-88 reduced 53.34% and 64.64% of DNMT3a expression in TQ2 QEFs, respectively. Subsequently the treatment of each siRNA reactivated enhanced green fluorescent protein(EGFP) expression in TQ2(224% and 114%). Our results might provide a clue for understanding the DNA methylation mechanism responsible for transgenic animal production and stable transgene expression.
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참고문헌 (24)
참고문헌 신청
Ballestar, E / 2008 / SnapShot: the human DNA methylome in health and disease / Cell 135 (6) : 1144 ~
Bernstein, B. E / 2007 / The mammalian epigenome / Cell 128 (4) : 669 ~ 681
Champagne, F. A / 2011 / Maternal imprints and the origins of variation / Horm Behav 60 (1) : 4 ~ 11
Chedin,F / 2011 / The DNMT3 Family of Mammalian De Novo DNA Methyltransferases / Prog Mol Biol Transl Sci 101 : 255 ~ 285
Chen, T / 2004 / Structure and function of eukaryotic DNA methyltransferases / Curr Top Dev Biol 60 : 55 ~ 89
Bernstein, B. E / 2007 / The mammalian epigenome / Cell 128 (4) : 669 ~ 681
Champagne, F. A / 2011 / Maternal imprints and the origins of variation / Horm Behav 60 (1) : 4 ~ 11
Chedin,F / 2011 / The DNMT3 Family of Mammalian De Novo DNA Methyltransferases / Prog Mol Biol Transl Sci 101 : 255 ~ 285
Chen, T / 2004 / Structure and function of eukaryotic DNA methyltransferases / Curr Top Dev Biol 60 : 55 ~ 89
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