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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

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학술저널
저자정보
저널정보
한국축산학회(구 한국동물자원과학회) 한국축산학회지 한국축산학회지 제55권 제4호
발행연도
2013.1
수록면
237 - 247 (11page)

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This study was to examine single or combined in vitro effects of environmental endocrine disruptors on boar sperm characteristics, oxidative stress damage in sperm and development of porcine IVF embryos. Addition of various concentration of NP(10, 20, 30μM), DBP(10, 50, 100 μM) and BPA(1, 5 or 10 μg/ml) on boar sperm characteristics such as percentages of sperm motility, viability, membrane integrity and mitochondrial activity were dose-dependently decreased within 3, 6 or 9 hr incubation period(p<0.05). The overall detrimental effects increased with incubation time increasement. NP, DBP and BPA showed the detrimental effects on sperm membrane and mitochondria of energy production organelles affecting cell viability with the dependancy of dose and incubation time. In combination effects, NP(10μM)+DBP(10μM) significantly decreased boar general sperm characteristics for 3 or 6 hr incubation period compared with control(p<0.05). When both of NP and DBP concentrations(NP; 30μM, DBP; 100μM) increase, the detrimental effects on sperm characteristics were larger than those of low concentration combination(p<0.05). The inhibitory effects of NP(30μM)+BPA(10μg/ml) on sperm characteristics were larger than those of NP(10μM)+BPA(1μg/ml)(p<0.05). DBP(100μM)+BPA(10μg/ml) decreased sperm characteristics compared with the low concentration combination(DBP 10μM +BPA 1μg/ml, p<0.05). This result indicates the detrimental effects of both chemicals on sperm characteristics were dose dependent. Addition of NP(30μM)+DBP(100μM), NP(30μM)+BPA(10μg/ml), DBP(10μM)+BPA(1μg/ml) or DBP(100μM)+BPA(10μg/ml) significantly increased lipid peroxidation for 3 or 6 hr incubation period(p<0.05) compared with no addition control. NP(≥20μM) decreased the percentages of IVF embryo development from morulae and blastocyst stages(p<0.05) and its detrimental effects were dose-dependant. BPA 0, 1, 5 or 10μg/ml decreased significantly and dose-dependently the percentage of morulae plus and blastocysts(p<0.05). Combinations of DBP(100μM) plus NP(30μM) and DBP(100μM) plus BPA(10μg/ml) did not affect on morulae and blastocyst development, but NP(30μM) plus BPA(10μg/ml) has significant detrimental effect on embryo development at these stages(p<0.05). These overall results indicate that the partial detrimental effects on boar sperm characteristics and embryo development by NP, DBP, BPA or the combination of these chemicals might be due to the increasement of lipid peroxidation and free radical formation in the cell and there were no specific interaction effects on boar sperm and embryo degeneration among the combined treatments.
#NP. DBP #BPA #Endocrine disruptors #Sperm characteristics #IVF embryo development

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참고문헌 (23)

참고문헌 신청
Akingbemi, BT / 2004 / Phthalate induced leydig cell hyperplasia associated with multiple endocrine disturbances / Proc Natl Acad Sci USA 101 : 775 ~ 780 google schola Bao, AM / 2011 / Effects of di-n-butyl phthalate on male rat reproduction following pubertal exposure / Asian J Andro 13 : 702 ~ 709 google schola Bolt, HM / 2001 / Comparative assessment of endocrine modulators with oestrogenic activity: I. Definition of a hygiene-based margein of safety(HBMOS) for xeno-oestrogens agai google schola Boockfor, FR / 1997 / Chronic administration of 4-tert-octylphenol to adult male rats causes shrinkage of the testes and male accessory sex organs, disrupts spermatogenesis, google schola Braun, JM / 2012 / Variability of urinary phthalate metabolite and bisphenol A concentrations before and during pregnancy / Environ Health Perspect 120 : 739 ~ 745 google schola

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