지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 1 - 5 (5page)
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As an alternative to radioimmunoassay a simple and highly sensitive enzyme immunoassay (EIA) was developed and validated for androstenedione quantification in plasma of Karan Fries bulls using second antibody coating technique. The wells of the microtitreplate were coated with affinity-purified goat immunoglobulin (antirabbit IgG) that binds the hormone specific antibody. The EIA was performed to analyze androstenedione directly in 40 μl of bull plasma. The androstenedione standards ranged from 0.20 to 200 pg/40 μl /well and the sensitivity of the assay was 5 pg/ml plasma. Serially diluted bull plasma containing high endogenous androstenedione showed good parallelism with bovine androstenedione standard curve. Intra- and inter-assay coefficients of variation (CV) were found to be 8 and 9%, respectively. Peripheral plasma androstenedione concentrations determined in young and adult bull samples ranged between 104–990 pg/ml and 184–2040 pg/ml, respectively.
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참고문헌 (13)
참고문헌 신청
Abdel Malak Bedair G / 1979 / Peripheral plasma androstenedione and testosterone concentrations in bulls before and during puberty / J Reprod Fertil 56 : 7 ~ 10
Ananadlaxmi N / 2001 / Production and purification of goat antirabbit IgG / Indian J Dairy Sci 54 : 332 ~ 334
Cook SJ / 1982 / Quantitation of six androgens by combined high performance liquid chromatography and radioimmunoassay / Steroids 40 : 369 ~ 380
Illera JC / 2003 / Amplified androstenedione enzyme immunoassay for the diagnosis of cryptorchidism in the male horse : comparison with testosterone and estrone sulfate metho
Kiser TE / 1978 / Comparison of testosterone and androstenedione secretion in bulls given prostaglandin F2α or luteinizing hormone / J Anim Sci 46 : 436 ~ 442
Ananadlaxmi N / 2001 / Production and purification of goat antirabbit IgG / Indian J Dairy Sci 54 : 332 ~ 334
Cook SJ / 1982 / Quantitation of six androgens by combined high performance liquid chromatography and radioimmunoassay / Steroids 40 : 369 ~ 380
Illera JC / 2003 / Amplified androstenedione enzyme immunoassay for the diagnosis of cryptorchidism in the male horse : comparison with testosterone and estrone sulfate metho
Kiser TE / 1978 / Comparison of testosterone and androstenedione secretion in bulls given prostaglandin F2α or luteinizing hormone / J Anim Sci 46 : 436 ~ 442
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