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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.9
- 수록면
- 275 - 281 (7page)
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This study was performed to characterize the chromosomal metallo-β-lactamases (MBLs) of Chryseobacterium indologenes isolated from Korea and to propose a clustering method of IND MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. indologenes. Nucleotide sequencing was performed by the dideoxy chain termination method using these PCR products. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. indologenes isolates contained all bla<SUB>IND</SUB> genes. DNA sequence analysis revealed that E. indologenes isolates possessed ten types of bla<SUB>IND</SUB> gene, including seven novel variants (bla<SUB>IND-8</SUB> to bla<SUB>IND-14</SUB>). The most common combination of MBL was IND-2 (n = 18). Minimum inhibitory concentrations of imipenem and meropenem for the isolates harboring novel IND MBLs were ≥ 16 μg/mL. IND MBLs were grouped in three clusters, based on amino acid similarities.
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재료 및 방법
결과
고찰
REFERENCES
참고문헌 (21)
참고문헌 신청
Atıcı S / 2016 / Ventilator-associated pneumonia caused by Chryseobacterium indologenes: a rare infant case and review of the literature 5:1741
Bayraktar MR / 2007 / Postoperative Chryseobacterium indologenes bloodstream infection caused by contamination of distillate water / Infect Control Hosp Epidemiol 28:368~369
Bellais S / 1999 / Molecular characterization of a carbapenem-hydrolyzing β-lactamase from Chryseobacterium (Flavobacterium) indologenes / FEMS Microbiol Lett 171:127~132
Bellais S / 2000 / Genetic diversity of carbapenem-hydrolyzing metallo-β-lactamases from Chryseobacterium (Flavobacterium) indologenes / Antimicrob Agents Chemother 44:3028~3034
Clinical and Laboratory Standards Institute / 2017 / Performance Standards for Antimicrobial Susceptibility Tests; approved standards M2-A8, 27th ed / CLSI
Bayraktar MR / 2007 / Postoperative Chryseobacterium indologenes bloodstream infection caused by contamination of distillate water / Infect Control Hosp Epidemiol 28:368~369
Bellais S / 1999 / Molecular characterization of a carbapenem-hydrolyzing β-lactamase from Chryseobacterium (Flavobacterium) indologenes / FEMS Microbiol Lett 171:127~132
Bellais S / 2000 / Genetic diversity of carbapenem-hydrolyzing metallo-β-lactamases from Chryseobacterium (Flavobacterium) indologenes / Antimicrob Agents Chemother 44:3028~3034
Clinical and Laboratory Standards Institute / 2017 / Performance Standards for Antimicrobial Susceptibility Tests; approved standards M2-A8, 27th ed / CLSI
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