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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 496 - 501 (6page)
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초록· 키워드
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Conventionally, immunotoxins have been produced as a single polypeptide from fused genes of an antibody fragment and a toxin. In this study, we adopted a unique approach of chemical conjugation of a toxin protein and an antibody fragment. The two genes were separately expressed in Escherichia coli and purified to high levels of purity. The two purified proteins were conjugated using a chemical linker. The advantage of this approach is its ability to overcome the problem of low recombinant immunotoxin production observed in some immunotoxins. Another advantage is that various combinations of immunotoxins can be prepared with fewer efforts, because the chemical conjugation of components is relatively simpler than the processes involved in cloning, expression, and purification of multiple immunotoxins. As a proof of concept, the scFv of trastuzumab and the PE24 fragment of Pseudomonas exotoxin A were separately produced using E. coli and then chemically crosslinked. The new immunotoxin was tested on four breast cancer cell lines variably expressing HER2. The chemically crosslinked immunotoxin exhibited cytotoxicity in proportion to the expression level of HER2. In conclusion, the present study revealed an alternative method of generating an immunotoxin that could effectively reduce the viability of HER2-expressing breast cancer cells. These results suggest the effectiveness of this method of immunotoxin crosslinking as a suitable alternative for producing immunotoxins.
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참고문헌 (30)
참고문헌 신청
Mathew M / 2009 / Humanized immunotoxins : a new generation of immunotoxins for targeted cancer therapy / Cancer Sci 100:1359~1365
Yamaizumi M / 1978 / One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell / Cell 15:245~250
Antignani A / 2013 / Immunotoxins : the role of the toxin / Toxins 5:1486~1502
Goldhirsch A / 2011 / Strategies for subtypes--dealing with the diversity of breast cancer : highlights of the St. Gallen International Expert Consensus on the Primary Therapy of Early Breast Cancer 2011 / Ann Oncol 22:1736~1747
Perou CM / 2000 / Molecular portraits of human breast tumours / Nature 406:747~752
Yamaizumi M / 1978 / One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell / Cell 15:245~250
Antignani A / 2013 / Immunotoxins : the role of the toxin / Toxins 5:1486~1502
Goldhirsch A / 2011 / Strategies for subtypes--dealing with the diversity of breast cancer : highlights of the St. Gallen International Expert Consensus on the Primary Therapy of Early Breast Cancer 2011 / Ann Oncol 22:1736~1747
Perou CM / 2000 / Molecular portraits of human breast tumours / Nature 406:747~752
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