지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
American foulbrood (AFB) is caused by the bacterium Paenibacillus larvae, which is highly contagious and often lethal to honeybee broods. To control AFB, rapid diagnostic tools including those based on immunological methods are required. We produced several specific mouse monoclonal antibodies (MAbs) against P. larvae. Interestingly, a few of the MAbs were revealed to be an IgM-type antibody. To ascertain the effects of adjuvants on immunoglobulin isotype switching, BALB/c mice were immunized with various adjuvants, i.e., Freund's adjuvant (FA), Alum adjuvant, and AddaVax™ followed by the generation of hybridoma that secreted monoclonal antibodies to P. larvae. In the case of AddaVax™, all screened hybridoma clones secreted IgG-type MAbs, whereas hybridomas generated by Alum and FA secreted 91.25% (7/80) and 66.67% (11/33) respectively, IgG-type MAbs. Although the mechanism of incomplete immunoglobulin isotype switching associated with the P. larvae antigen needs further study, our results indicate that the applied adjuvants can have a significant effect on immunoglobulin isotype switching results.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Apiratmateekul N / 2009 / A modified hybridoma technique for production of monoclonal antibodies having desired isotypes / Cytotechnol 60:45~51
Awate S / 2013 / Mechanisms of action of adjuvants / Front Immunol 4:114
Bamrick JF / 1967 / Resistance to american foulbrood in honey bees: VI. Spore germination in larvae of different ages / J Invertebr Pathol 9:30~34
Buchmann SL / 1996 / The conservation of bees / Academic Press :125~142
Ellis JD / 2005 / The worldwide health status of honey bees / Bee World 86:88~101
Awate S / 2013 / Mechanisms of action of adjuvants / Front Immunol 4:114
Bamrick JF / 1967 / Resistance to american foulbrood in honey bees: VI. Spore germination in larvae of different ages / J Invertebr Pathol 9:30~34
Buchmann SL / 1996 / The conservation of bees / Academic Press :125~142
Ellis JD / 2005 / The worldwide health status of honey bees / Bee World 86:88~101
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