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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제7호
- 발행연도
- 2019.1
- 수록면
- 1,117 - 1,123 (7page)
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초록· 키워드
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Control of pine wilt disease, which is caused by pine wilt nematode Bursaphelenchus xylophilus, is heavily dependent on the use of chemicals such as abamectin. Although such chemicals are highly effective, demands for alternatives that are derived preferentially from natural sources, are increasing out of environmental concerns. One of the challenges to discovery of alternative control agents is lack of fast and efficient screening method that can be used in a highthroughput manner. Here we described the development of colorimetric assay for the rapid and accurate screening of candidate nematicidal compounds/biologics targeting B. xylophilus. Contrary to the conventional method, which relies on laborious visual inspection and counting of nematode population under microscope, our method utilizes a redox dye that changes its color in response to metabolic activity of nematode population in a given sample. In this work, we optimized parameters of our colorimetric assay including number of nematodes and amount of redox dye, and tested applicability of our assay for screening of chemicals and biologics. We demonstrated that our colorimetric assay can be applied to rapid and accurate quantification of nematode viability/mortality in a nematode population treated with candidate chemicals/biologics. Application of our method would facilitate highthroughput endeavors aiming at finding environment-friendly control agents for deadly disease of pine trees.
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참고문헌 (18)
참고문헌 신청
Mota MM / 2008 / Pine wilt disease: a worldwide threat to forest ecosystems / Springer :1~14
Yano S / 1913 / Investigation on pine death in Nagasaki prefecture / Sanrin-Kouhou 4:1~14
Steiner G / 1932 / Aphelenchoides xylophilus, a nematode associated with blue-stain and other fungi in timber / J. Agric. Res 48:949~951
Evans HF / 1996 / Pest risk analysis(PRA)for the territories of the European Union(as PRA area)on Bursaphelenchus xylophilus and tis vector in the genus Monochamus / EPPO Bulletin 26:199~249
Jikumaru S / 2001 / Transmission of Bursaphelenchus mucronatus(Nematoda : Aphelenchoididae)through feeding wounds by Monochamus saltuarius(Coleoptera : Cerambycidae) / Nematology 3:325~333
Yano S / 1913 / Investigation on pine death in Nagasaki prefecture / Sanrin-Kouhou 4:1~14
Steiner G / 1932 / Aphelenchoides xylophilus, a nematode associated with blue-stain and other fungi in timber / J. Agric. Res 48:949~951
Evans HF / 1996 / Pest risk analysis(PRA)for the territories of the European Union(as PRA area)on Bursaphelenchus xylophilus and tis vector in the genus Monochamus / EPPO Bulletin 26:199~249
Jikumaru S / 2001 / Transmission of Bursaphelenchus mucronatus(Nematoda : Aphelenchoididae)through feeding wounds by Monochamus saltuarius(Coleoptera : Cerambycidae) / Nematology 3:325~333
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