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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제3호
- 발행연도
- 2019.1
- 수록면
- 392 - 400 (9page)
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초록· 키워드
오류제보하기
Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum. CgkfoC and CgkfoA genes with host codon preference were synthesized and driven by promoter Ptac, which was confirmed as a strong promoter via GFPuv reporter assessment. In a lactate dehydrogenase (ldh) deficient host, intracellular chondroitin titer increased from 0.25 to 0.88 g/l compared with that in a wild-type host. Moreover, precursor enhancement via overexpressing precursor synthesizing gene ugdA further improved chondroitin titers to 1.09 g/l. Chondroitin production reached 1.91 g/l with the engineered strain C. glutamicum ΔL-CgCAU in a 5-L fed-batch fermentation with a single distribution Mw of 186 kDa. This work provides an alternative, safe and novel means of producing chondroitin for industrial applications.
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참고문헌 (35)
참고문헌 신청
Vázquez JA / 2013 / Chondroitin sulfate, hyaluronic acid and chitin/chitosan production using marine waste sources : characteristics, applications and ecofriendly processes : a review / Mar. Drugs 11:747~774
Schiraldi C / 2010 / Production of chondroitin sulfate and chondroitin / Appl. Microbiol. Biotechnol 87:1209~1220
Roma n E / 2003 / Overexpression of udp-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of k5 polysaccharide / Biochem. J 374:767~772
Kwok JC / 2012 / Chondroitin sulfate : a key molecule in the brain matrix / Int. J. Biochem. Cell Biol 44:582~586
Lauder R / 2009 / Chondroitin sulphate : a complex molecule with potential impacts on a wide range of biological systems / Complement. Ther. Med 17:56~62
Schiraldi C / 2010 / Production of chondroitin sulfate and chondroitin / Appl. Microbiol. Biotechnol 87:1209~1220
Roma n E / 2003 / Overexpression of udp-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of k5 polysaccharide / Biochem. J 374:767~772
Kwok JC / 2012 / Chondroitin sulfate : a key molecule in the brain matrix / Int. J. Biochem. Cell Biol 44:582~586
Lauder R / 2009 / Chondroitin sulphate : a complex molecule with potential impacts on a wide range of biological systems / Complement. Ther. Med 17:56~62
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