Genetic Diversity of Plasmodium vivax in Clinical Isolates from Southern Thailand using PvMSP1, PvMSP3 (PvMSP3α, PvMSP3β) Genes and Eight Microsatellite Markers

Supinya Thanapongpichat; Thunchanok Khammanee; Nongyao Sawangjaroen; Hansuk Buncherd; Aung Win Tun

추천

검색

질문

자료유형: 학술저널

저자정보: Supinya Thanapongpichat (Prince of Songkla University) Thunchanok Khammanee (Prince of Songkla University) Nongyao Sawangjaroen (Prince of Songkla University) Hansuk Buncherd (Prince of Songkla University) Aung Win Tun (Mahidol University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.57 No.5

발행연도: 2019.10

수록면: 469 - 479 (11page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Plasmodium vivax is usually considered morbidity in endemic areas of Asia, Central and South America, and some part of Africa. In Thailand, previous studies indicated the genetic diversity of P. vivax in malaria-endemic regions such as the western part of Thailand bordering with Myanmar. The objective of the study is to investigate the genetic di¬versity of P. vivax circulating in Southern Thailand by using 3 antigenic markers and 8 microsatellite markers. Dried blood spots were collected from Chumphon, Phang Nga, Ranong and, Surat Thani provinces of Thailand. By PCR, 3 distinct sizes of PvMSP3α, 2 sizes of PvMSP3β and 2 sizes of PvMSP1 F2 were detected based on the length of PCR products, respectively. PCR/RFLP analyses of these antigen genes revealed high levels of genetic diversity. The genotyping of 8 mi-crosatellite loci showed high genetic diversity as indicated by high alleles per locus and high expected heterozygosity (H<SUB>E</SUB>). The genotyping markers also showed multiple-clones of infection. Mixed genotypes were detected in 4.8% of PvMSP3α, 29.1% in PvMSP3β and 55.3% of microsatellite markers. These results showed that there was high genetic diversity of P. vivax isolated from Southern Thailand, indicating that the genetic diversity of P. vivax in this region was comparable to those observed other areas of Thailand.

#Plasmodium vivax #malaria #genetic diversity #antigenic marker #microsatellite marker

참고문헌 (49)

참고문헌 신청

World Health Organization / 2016 / World Malaria Report 2016

World Health Organization / 2015 / World Malaria Report 2015

Wongsrichanalai C / 2008 / Declining artesunate-mefloquine efficacy against falciparum malaria on the Cambodia-Thailand border / Emerg Infect Dis 14:716~719

Rijken MJ / 2011 / Chloroquine resistant vivax malaria in a pregnant woman on the western border of Thailand / Malar J 10:113

Howes RE / 2012 / G6PD deficiency prevalence and estimates of affected populations in malaria endemic countries : a geostatistical model-based map / PLoSMed 9:e1001339

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!