Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors

Hye-Jung Kim; Hye-Jin Ahn; Hyeweon Kang; Jaehui Park; Seul gi Oh; Saehae Choi; Won-Kyu Lee; Ho-Woo Nam

추천

검색

질문

자료유형: 학술저널

저자정보: Hye-Jung Kim (OSONG Biomedical Innovation Foundation) Hye-Jin Ahn (Catholic University of Korea) Hyeweon Kang (OSONG Biomedical Innovation Foundation) Jaehui Park (OSONG Biomedical Innovation Foundation) Seul gi Oh (OSONG Biomedical Innovation Foundation) Saehae Choi (OSONG Biomedical Innovation Foundation) Won-Kyu Lee (OSONG Biomedical Innovation Foundation) Ho-Woo Nam (Catholic University of Korea)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.58 No.3

발행연도: 2020.6

수록면: 249 - 255 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Toxoplasma gondii, a ubiquitous, intracellular parasite of the phylum Apicomplexa, infects an estimated one-third of the human population as well as a broad range of warm-blooded animals. We have observed that some tyrosine kinase inhibitors suppressed the growth of T. gondii within host ARPE-10 cells. Among them, afatinib, human epithermal growth factor receptor 2 and 4 (HER2/4) inhibitor, may be used as a therapeutic agent for inhibiting parasite growth with minimal adverse effects on host. In this report, we conducted a proteomic analysis to observe changes in host proteins that were altered via infection with T. gondii and the treatment of HER2/4 inhibitors. Secreting proteins were subjected to a procedure of micor basic reverse phase liquid chromatography, nano-liquid chromatography-mass spectrometry, and ingenuity pathway analysis serially. As a result, the expression level of heterogeneous nuclear ribonucleoprotein K, semaphorin 7A, a GPI membrane anchor, serine/threonine-protein phosphatase 2A, and calpain small subunit 1 proteins were significantly changed, and which were confirmed further by western blot analysis. Changes in various proteins, including these 4 proteins, can be used as a basis for explaining the effects of T. gondii infections and HER2/4 inhibitors.

#Toxoplasma gondii #HER2/4 inhibitors #secretome #semaphorin 7A #calpain small subunit 1

참고문헌 (28)

참고문헌 신청

Tenter AM / 2000 / Toxoplasma gondii : from animals to humans / Int J Parasitol 30:1217~1258

Jackson MH / 1989 / The prevalence and source of Toxoplasma infection in the environment / Adv Parasitol 28:55~105

Chung WH / 2008 / Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis / Nat Med 14:1343~1350

Decoster A / 1988 / Recognition of Toxoplasma gondii excreted and secreted antigens by human sera from acquired and congenital toxoplasmosis : identification of markers of acute and chronic infection / Clin Exp Immunol 73:376~382

Darcy F / 1988 / Induction of a protective antibody-dependent response against toxoplasmosis by in vitro excreted/secreted antigens from tachyzoites of Toxoplasma gondii / Parasite Immunol 10:553~567

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!