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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제1호
- 발행연도
- 2020.1
- 수록면
- 44 - 53 (10page)
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Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 μg/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 1010 CFU/ml to 1012 CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.
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참고문헌 (42)
참고문헌 신청
Vavrusova M / 2014 / Calcium nutrition. Bioavailability and fortification / LWT - Food Sci. Technol. 59:1198~1204
Vavrusova M / 2018 / Codissolution of calcium hydrogenphosphate and sodium hydrogencitrate in water. Spontaneous supersaturation of calcium citrate increasing calcium bioavailability / J. Food Drug Anal. 26:330~336
Titchenal CA / 2007 / A system to assess the quality of food sources of calcium / J. Food Compost Anal 20:717~724
Camara-Martos F / 2002 / Influence of dietary factors on calcium bioavailability / Biol. Trace Elem. Res 89:43~52
Bolland MJ / 2011 / Calcium supplements with or without vitamin D and risk of cardiovascular events: reanalysis of the Women’s Health Initiative limited access dataset and meta-analysis / BMJ 342:d2040
Vavrusova M / 2018 / Codissolution of calcium hydrogenphosphate and sodium hydrogencitrate in water. Spontaneous supersaturation of calcium citrate increasing calcium bioavailability / J. Food Drug Anal. 26:330~336
Titchenal CA / 2007 / A system to assess the quality of food sources of calcium / J. Food Compost Anal 20:717~724
Camara-Martos F / 2002 / Influence of dietary factors on calcium bioavailability / Biol. Trace Elem. Res 89:43~52
Bolland MJ / 2011 / Calcium supplements with or without vitamin D and risk of cardiovascular events: reanalysis of the Women’s Health Initiative limited access dataset and meta-analysis / BMJ 342:d2040
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