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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제6호
- 발행연도
- 2020.1
- 수록면
- 868 - 877 (10page)
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초록· 키워드
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Bicarbonate has been considered as a better approach for supplying CO2 to microalgae cells microenvironments than gas bubbling owing to cost-effectiveness and easy operation. However, the β-carotene production was too low in Dunaliella salina cultivated with bicarbonate in previous studies. Also, the difference in photosynthetic efficiency between these two carbon sources (bicarbonate and CO2) has seldom been discussed. In this study, the culture conditions, including NaHCO3, Ca2+, Mg2+ and microelement concentrations, were optimized when bicarbonate was used as carbon source. Under optimized condition, a maximum biomass concentration of 0.71 g/l and corresponding β-carotene content of 4.76% were obtained, with β-carotene yield of 32.0 mg/l, much higher than previous studies with NaHCO3. Finally, these optimized conditions with bicarbonate were compared with CO2 bubbling by online monitoring. There was a notable difference in Fv/Fm value between cultivations with bicarbonate and CO2, but there was no difference in the Fv/Fm periodic changing patterns. This indicates that the high concentration of NaHCO3 used in this study served as a stress factor for β-carotene accumulation, although high productivity of biomass was still obtained.
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참고문헌 (31)
참고문헌 신청
Bonnefond H / 2016 / Concomitant effects of light and temperature diel variations on the growth rate and lipid production of Dunaliella salina / Alga. Res 14:72~78
Bonnefond H / 2017 / Coupling and uncoupling of triglyceride and betacarotene production by Dunaliella salina under nitrogen limitation and starvation / Biotechnol. Biofuels 10:25
Gong M / 2016 / Carotenoids from microalgae : a review of recent developments / Biotechnol. Adv 34:1396~1412
Chekanov K / 2017 / Effects of CO2 enrichment on primary photochemistry, growth and astaxanthin accumulation in the chlorophyte Haematococcus pluvialis / J. Photochem. Photobiol. B 171:58~66
Doucha J / 2005 / Utilization of flue gas for cultivation of microalgae (Chlorella sp.) in an outdoor open thin-layer photobioreactor / J. Appl. Phycol. 17:403~412
Bonnefond H / 2017 / Coupling and uncoupling of triglyceride and betacarotene production by Dunaliella salina under nitrogen limitation and starvation / Biotechnol. Biofuels 10:25
Gong M / 2016 / Carotenoids from microalgae : a review of recent developments / Biotechnol. Adv 34:1396~1412
Chekanov K / 2017 / Effects of CO2 enrichment on primary photochemistry, growth and astaxanthin accumulation in the chlorophyte Haematococcus pluvialis / J. Photochem. Photobiol. B 171:58~66
Doucha J / 2005 / Utilization of flue gas for cultivation of microalgae (Chlorella sp.) in an outdoor open thin-layer photobioreactor / J. Appl. Phycol. 17:403~412
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