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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 45 - 53 (9page)
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초록· 키워드
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Centella asiatica contains various triterpenoids, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. Among them, they are mainly contained in leaf, and among them asiaticoside is known as tissue-specific compound that are mainly accumulated in the leaves. Therefore, in the present study, C. asiatica hairy root cultures were established from petiole and leaf explants using Agrobacterium rhizogenes-mediated transformation. To promote the biosynthesis of these triterpenoids, hairy root cultures were treated with precursors (squalene and pyruvic acid) and elicitors [methyl jasmonate (MeJA) and salicylic acid (SA)] at various concentrations. No significant difference was detected in the growth index of hairy root cultures between precursor feeding and elicitation, except pyruvic acid feeding, suggesting that elicitors do not affect the growth of hairy roots. Treatment with 2.5 mM squalene and 5 mM pyruvic acid enhanced the biosynthesis of target triterpenoids (57.53 and 29.13 mg g−1 DW, respectively) compared with other treatments. However, compared to squalene, MeJA showed a greater effect on the triterpene saponin content in C. asiatica. The highest quantity of triterpenoids (60.25 mg g−1 DW) was produced in hairy root cultures treated with 400 μM MeJA. These results indicate that precursorand elicitor-treated C. asiatica hairy root cultures rapidly accumulate biomass and could be used to enhance the production of triterpenoids for industrial purposes.
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참고문헌 (44)
참고문헌 신청
Bohm H / 2004 / Betaxanthin formation and free amino acids in hairy roots of Beta vulgaris var. lutea depending on nutrient medium and glutamate feeding / Phytochem 65:1361~1368
Bonfill M / 2011 / Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica / Plant Cell Tiss Org 104:61~67
Chavan SP / 2011 / Influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of Carthamus tinctorius L / Appl Microbiol Biotechnol 89:1701~1707
Devkota A / 2010 / Centella asiatica (L.) urban from Nepal: quali-quantitative analysis of samples from several sites, and selection of high terpene containing populations for cultivation / Biochem Syst Ecol 38:12~22
Durner J / 1997 / Salicylic acid and disease resistance in plants / Trends Plant Sci 2:7
Bonfill M / 2011 / Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica / Plant Cell Tiss Org 104:61~67
Chavan SP / 2011 / Influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of Carthamus tinctorius L / Appl Microbiol Biotechnol 89:1701~1707
Devkota A / 2010 / Centella asiatica (L.) urban from Nepal: quali-quantitative analysis of samples from several sites, and selection of high terpene containing populations for cultivation / Biochem Syst Ecol 38:12~22
Durner J / 1997 / Salicylic acid and disease resistance in plants / Trends Plant Sci 2:7
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