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자료유형
학술대회자료
저자정보
Bian, Yinbing (Institute of applied mycology, plant science and technology college, Huazhong Agricultural University) Xiong, Xuan (Institute of applied mycology, Plang Science and technology college, Huazhong Agricultural University)
저널정보
한국균학회 한국균학회 학술대회 한국균학회 2009년도 추계학술대회 및 정기총회
발행연도
2009.1
수록면
14 - 16 (3page)

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Poria cocos is an importantant medical macrofungus,the sclerotium of Poria cocos has specific value as the drug material. There are few papers about its breeding and spawn rejuvenation. In this project, the protoplasts of cultivated strain T and wild strain L were prepared and treated separately by ultraviolet and heating, then fused with the PEG6000. The tural fusants were selected and identified by the affinity and ISSR analysis. 71 incompatibility strains between parents and reg regenerations were obtained from 118 regenerations by the affinity analysis. Five incompatibility strains were amplified with different primers, the results were showed that they had specific bands of both parents in the profile amplified with 3 primers, which proved these 5 strains were fusants by means of molecular biology marker. On the other hand, 25 strain were selected from 168 protoplast regenerations of cultivated strain T for cultivation experiment. The fresh sclerotium weight of these protoplast regenerations were better than the original strain.significantil 3 strains (T-1, T-4, T-7) increased respectively 118%, 73% and 73% than original strain. This method could be the effective in the rejuvenation Poria cocos.

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