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자료유형
학술저널
저자정보
Park, Ju-Yong (Division of Forest Diseases and Insect Pests, Korea Forest Research Institute) Shin, Keum-Chul (Tree Pathology and Mycology Laboratory, Kangwon National University) Kim, Kyung-Hee (Division of Forest Diseases and Insect Pests, Korea Forest Research Institute) Lee, Seung Kyu (Division of Forest Diseases and Insect Pests, Korea Forest Research Institute) Lee, Jong Kyu (Tree Pathology and Mycology Laboratory, Kangwon National University)
저널정보
한국산림과학회(구 한국임학회) 한국산림과학회지 한국산림과학회지 제94권 제5호
발행연도
2005.1
수록면
334 - 337 (4page)

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Pine wood nematode isolates from Haman, Gyoungsangnam-do were cryopreserved with three different cryoprotectants (glycerol, ethylene glycol and dimethylsulfoxide), Nematode Growth medium (NGM) and M 9 buffer solution, respectively, and then survival rate, reproduction ability, and pathogenicity of stored nematodes were compared. Survival rates of juvenile was excellent in 15% glycerol solution as $72{\pm}5.3%$. The survival ability of nematodes at juvenile stage was more efficient than that of nematodes at adult stage. Pre-incubation treatment at $4^{\circ}C$ before storing in liquid nitrogen didn't affect the survival ability of pine wood nematode. When the reproduction ability of nematodes was confirmed on Botrytis cinerea agar plates at $28^{\circ}C$ incubator, there was no difference between cryopreserved and non-cryopreserved. Pathogenic ability of cryopreserved nematodes was also evaluated and confirmed by artificially inoculating them on 2-year old red pine seedlings.

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