Optimization of Culture Conditions for Production of Pneumococcal Capsular Polysaccharide Type IV

Kim, S.N.; Min, K.K.; Choi, I.H.; Kim, S.W.; Pyo, S.N.; Rhee, D.K.

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자료유형: 학술저널

저자정보: Kim, S.N. (College of Pharmacy, Sungkyunkwan University) Min, K.K. (College of Pharmacy, Sungkyunkwan University) Choi, I.H. (College of Pharmacy, Sungkyunkwan University) Kim, S.W. (College of Pharmacy, Sungkyunkwan University) Pyo, S.N. (College of Pharmacy, Sungkyunkwan University) Rhee, D.K. (College of Pharmacy, Sungkyunkwan University)

저널정보: 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제19권 제3호

발행연도: 1996.1

수록면: 173 - 177 (5page)

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The Pneumococcus, Streptococcus pneumoniae, has an ample polysaccharide (PS) capsule that is highly antigenic and is the main virulence factor of the organism. The capsular PS is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsular PS by type 4 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based medium were found to support luxuriant growth of pneumococcus type 4 at the same level. Therefore in this study, the Casitone based medium was used to study optimization of the culture condition because of BHI broth's high cost and complex nature. The phase of growth which accomodated maximum PS production was exponential phase. Concentrations of glucose greater than 0.8% did not enhance growth or PS production. Substitution of nitrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium, copper, and magnesium ions) had adverse effects on growth and PS production. On the other hand, low level aeration and supplementation of 3 mg/l concentration of asparagine, phenylalanine, or threonine were beneficial for increased PS production. The synergistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cumulative increase in capsular PS production.

#S #pneumoniae type 4 #Casitone meduim #Polyascchride #Culture optimization

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