Carbon-Partitioning in Arabidopsis Is Regulated by the Fructose 6-Phosphate, 2-Kinase/Fructose 2,6-Bisphosphatase Enzyme

Lee Youn Hyung; Lee Dong Seok; Lim Jong Min; Yoon Joo Mi; Bhoo Seong Hee; Jeon Jong Seong; Hahn Tae Ryong

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자료유형: 학술저널

저자정보: Lee Youn Hyung (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) Lee Dong Seok (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) Lim Jong Min (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) Yoon Joo Mi (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) Bhoo Seong Hee (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) Jeon Jong Seong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) Hahn Tae Ryong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University)

저널정보: 한국식물학회 식물학회지 식물학회지 제49권 제1호

발행연도: 2006.1

수록면: 70 - 79 (10page)

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To further elucidate the mechanisms underlying carbon-partitioning in plants, we established an experimental system by generating transgenic Arabidopsis lines that overexpress both the fructose 6-phosphate, 2-kinase (F6P,2-K) and the fructose 2,6-bisphosphatase (F26BPase) domains. We also produced knockout transgenic plants for these domains via RNAi and T-DNA tagging. In F6p,2-K overexpressing transgenics, F6p'2-K activity increased slightly and $Fru-2,6-P_2$ levels were elevated by $80\%$, compared with the wild type (WT). F26BPase activity was similar between the WT and transgenic plants. However, when that domain was overexpressed, F26BPase activity was increased by $70\%$ compared with the WT, whereas F6p,2-K activity was reduced to $85\%$ of the WT level. In knockout and RNAi mutant lines that showed reduced F6p,2-K and F26BPase activities, levels of $Fru-2,6-P_2$ were only between 3 to $7\%$ of those for the WT: In F6p,2-K overexpressing transgenic lines, the levels of starch, hexose, and triose phosphates slightly increased, while sucrose content was marginally reduced. In F26BPase overexpressing plants, however, the levels of soluble sugars and hexose phosphates were slightly increased, but starch and triose phosphate contents declined. Furthermore, compared with the wr, the levels of soluble sugars rose while starch and hexose phosphate quantities decreased in 2-kinase/fructose-2,6-bisphophatase knockout mutants. Therefore, our data reaffirms that $Fru-2,6-P_2$ contributes to the regulation of photosynthetic carbon-partitioning between starch and sucrose in Arabidopsis leaves by limiting sucrose synthesis.

#Arabidopsis #carbohydrate metabolites #carbon-partitioning, fructose 2,6-bisphosphate #fructose 6-phosphate #2-kinase/fructose 2,6-bisphosphatase

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