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자료유형
학술저널
저자정보
Kim, Dong-Hyun (College of Pharmacy, Kyung Hee University) Kim, Byung-Taek (College of Pharmacy, Kyung Hee University) Park, Sun-Yong (College of Pharmacy, Kyung Hee University) Kim, Na-Young (Department of Food and Nutrition, Kyung Hee University) Han, Myung-Joo (Department of Food and Nutrition, Kyung Hee University) Shin, Kuk-Hyun (Natural Products Research Institute, Seoul National University) Kim, Wan-Suk (Natural Products Research Institute, Seoul National University) Kim, Yeong-Sik (Natural Products Research Institute, Seoul National University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제21권 제5호
발행연도
1998.1
수록면
576 - 580 (5page)

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When glycosaminoglycan (GAG)-degrating enzymes were measured in normal human stool suspensions, all 5 tested different stools degraded titrable heparin and acharan sulfate. GAG-degrading bacteria were screened from the isolates of human stools. Among them, HJ-15 had the most potent activities of heparinases (GAGs-degrading enzymes). However, HJ-15 produced the enzyme even if in the media without heparin. Acharan sulfate lyase was induced by acharan sulfate and heparin. Heparinase production was also induced by these GAGs. These enzymes, acharan sulfate lyase and heparinase, were produced in exponential and stationary phase of HJ-15 growth, respectively. Optimal pHs of the acharan sulfate lyase and heparinase activities were 7.2 and 7.5 respectively. the biochemical properties of HJ-15 was similar to those of B. stercoris. However, difference from B. stercoris was utilization of raffinose. this HJ-15 also degraded chondroitin sulfates A and C.

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