Dexamethasone Inhibits the Formation of Multinucleated Osteoclasts via Down-regulation of ${beta}_3$ Integrin Expression

Kim, Yong-Hee; Jun, Ji-Hae; Woo, Kyung-Mi; Ryoo, Hyun-Mo; Kim, Gwan-Shik; Baek, Jeong-Hwa

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자료유형: 학술저널

저자정보: Kim, Yong-Hee (Department of Cell and Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) Jun, Ji-Hae (Department of Cell and Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) Woo, Kyung-Mi (Department of Cell and Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) Ryoo, Hyun-Mo (Department of Cell and Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) Kim, Gwan-Shik (Department of Cell and Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University) Baek, Jeong-Hwa (Department of Cell and Developmental Biology, School of Dentistry and Dental Research Institute, Seoul National University)

저널정보: 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제29권 제8호

발행연도: 2006.1

수록면: 691 - 698 (8page)

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Although glucocorticoids are known to affect osteoclast differentiation and function, there have been conflicting reports about the effect of glucocorticoids on osteoclast formation, leading to the assumption that microenvironment and cell type influence their action. We explored the effect of the synthetic glucocorticoid analog dexamethasone on the formation of osteoclasts. Dexamethasone inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts without affecting the formation of TRAP-positive mononuclear cells in a coculture of mouse osteoblasts and bone marrow cells. Dexamethasone did not inhibit mRNA expression levels of the receptor activator of nuclear factor-kB ligand and osteoprotegerin, the essential regulators of osteoclastogenesis. Dexamethasone down-regulated the expression of ${\beta}_3$ integrin mRNA and protein but did not alter expression of other osteoclast differentiation marker genes. Both dexamethasone and echistatin, a ${\beta}_3$ integrin function blocker, inhibited TRAP-positive multinucleated osteoclast formation but not TRAP-positive mononuclear cell formation. These results suggest that dexamethasone inhibits the formation of multinucleated osteoclasts, at least in part, through the down-regulation of ${\beta}_3$ integrin, which plays an important role in the formation of multinucleated osteoclasts.

#Dexamethasone #Osteoclast #Vitronectin receptor #${beta}_3$ Integrin #Fusion

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이 논문의 저자 정보

Kim, Yong-Hee

소속기관 Department of Bioengineering, Hanyang University

주요연구분야 의약학 > 약학

논문수 5 이용수 0

Jun, Ji-Hae