지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 207 - 220 (14page)
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초록· 키워드
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Biofilm formation, attachment and cell hydrophobicity of foodborne pathogens, including Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus were investigated under various environmental conditions such as sodium chloride (0.5-7.0%, w/v), glucose (0.25-10.0%, w/v), pH (6.0-6.8), temperature (25 and $37^{\circ}C$), incubation time (24 and 6h), and nutrients trypic soy broth (TSB) and diluted TSB (1:10). Biofilm formation for 24 h at 25 and $37^{\circ}C$ and attachment for 30 min and 6 h on the surface of polystyrene were measured by the crystal violet staining method. Cell hydrophobicity of pathogens for 6 and 24 h at 25 and $37^{\circ}C$ was conducted using the modified bacterial adherence to hydrocarbons method (mBATH). Biofilm formation and attachment of pathogens were highly influenced by the addition of glucose and sodium chloride compared to pH. The biofilm of all pathogens formed in TSB was greater than that in diluted TSB. Biofilm formations of S. aureus and P. aeruginosa at $37^{\circ}C$ were greater than that at $25^{\circ}C$. However, biofilm formation of L. monocytogenes was not significantly affected by temperature. Levels of L. monocytogenes hydrophobicity were influenced by adding glucose and sodium chloride at $37^{\circ}C$, whereas levels of hydrophobicity for other pathogens were significantly different depending on the glucose condition (p<0.05). The results demonstrate that biofilm formation, attachment, and hydrophobicity of pathogens were affected by environmental conditions such as the addition of glucose and sodium chloride. However, factors affecting biofilm formation and cell hydrophobicity differed depending on the pathogen type.
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