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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Jong-Hwan (Department of Agricultural Biotechnology, Seoul National University) Seo, Jong-Su (Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology) Moon, Joon-Kwan (School of Plant Life and Environmental Sciences, Hankyong National University) Kim, Jeong-Han (Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제56권 제1호
발행연도
2013.1
수록면
47 - 54 (8page)

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초록· 키워드

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Multi-residue method of eight benzoylurea insecticdes (8BUs; diflubenzuron, triflumuron, hexaflumuron, teflubenzuron, novaluron, lufenuron, flufenoxuron, and chlorfluazuron) in apple and mandarin were developed with high performance liquid chromatography and liquid chromatography-tandem Mass spectrometry. Using multiresidue standard solutions, extraction, partition, and clean-up procedures have been established. Extraction with acetone and partition with n-hexane gave reasonable recovery of more than 90%. Clean-up with florisil-glass column using acetone/n-hexane as eluting solvents resulted in good efficiency of higher than 94%. By employing the established analytical method, recovery test of 8BUs in mandarin and apple was conducted to give recovery of 80-110%, which satisfies the Korea Food & Drug Administration criteria. The method limit of quantitation (MLOQ, 0.02 mg/kg) was lower than 1/2 of maximum residue limits of crops. Excellent sensitivity and selectivity were obtained with liquid chromatography tandem mass spectrometry (LC-MS/MS)-Electrospray ionization(-) even in the samples with no clean-up, suggesting LC-MS/MS can be used for the fast multi-residue method of 8 benzoylurea insecticides in mandarin and apple.

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