골유도재생술용 차폐막 적용을 위한 polyvinylidene fluoride와 polyacrylonitrile 나노섬유의 평가

임윤경; 국중기; 유상준; 이원표; 이경현; 김희중; 이승훈; 김찬

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자료유형: 학술저널

저자정보: 임윤경 (조선대학교 치과대학 구강생화학교실) 국중기 (조선대학교 치과대학 구강생화학교실) 유상준 (조선대학교 치과대학 치주과학교실) 이원표 (조선대학교 치과대학 치주과학교실) 이경현 (조선대학교 치과대학 치주과학교실) 김희중 (조선대학교 치과대학 보철학교실) 이승훈 (숭실대학교 유기신소재파이버공학과) 김찬 ([주]아모그린텍 신소재 연구소)

저널정보: 한국섬유공학회 한국섬유공학회지 한국섬유공학회지 제56권 제3호

발행연도: 2019.1

수록면: 125 - 134 (10page)

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Recently, various nanofiber membranes for guided bone regeneration using electrospinning methods have been reported. The purpose of this study was to evaluate the in vitro osteogenesis efficiency of electrospun polyvinylidene fluoride (PVDF) and polyacrylonitrile (PAN) membranes. Nano sized topography, mechanical properties, and biological evaluations were performed to compare the PVDF and PAN nanofiber membranes with the commercially available polytetrafluoroethylene (PTFE) membrane. The cytotoxicities of these membranes were evaluated using the MTT assay. Alkaline phosphatase (ALP) activity and the calcium concentration were determined to evaluate the in vitro bone formation activities of the above-mentioned membranes. Expression levels of the osteoblast differentiation marker genes (RUNX2, OSX, OCN, and COL1A1 genes) were determined using the real-time quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent (ELISA) assay. Approximately 300 nm three-dimensional porous structures, with pores connected/extending from the surface to the rear/bottom were observed in each PVDF and PAN nanofiber membrane using a scanning electron microscope. The strength and elongation of the porous structures were superior to those seen in the PTFE membrane. The cell viability, ALP activity, and calcium concentration were higher in these nanofiber membranes as compared to those in the PTFE membrane. RT-qPCR and ELISA assay analysis also showed that the nanofiber membranes had better osteogenesis efficiencies as compared to the PTFE membrane after 7 days. The results in this study demonstrate that the electrospun PVDF and PAN nanofiber membranes could potentially function as barrier membranes for bone tissue regeneration.

#bone regeneration #electrospinning #nanofibers #polyacrylonitrile #polyvinylidene fluoride #PTFE

참고문헌 (20)

참고문헌 신청

신찬수 / 2005 / 뼈의 재형성 및 무기질화 / Endocrinology and Metabolism 20(6):543~555

원영순 / 2014 / 한국 성인의 치주질환 위험 요인 / 대한구강보건학회지 38(3):176~183

A. H. Melcher / 1976 / On the Repair Potential of Periodontal Tissue / J. Periodontol 47:256~260

한대현 / 2008 / 실크 피브로인 나노 차폐막(나노가이드-에스)을 이용한 치조골 유도재생능력에 관한 비교 연구 / Journal of Periodontal & Implant Science 38(3):475~482

J. Gottlow / 1986 / New Attachment Formation in the Human Periodontium by Guided Tissue Regeneration. Case Reports / J. Clin. Periodontol 13:604~616

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